文献类型：期刊文献

英文题名：Mining marine dinoflagellate Amphidinium carterae for peridinin production by optimizing light conditions and mixotrophy strategy

作者：Hong, Zhiwei[1];Qiu, Sheng[1];E, Jiawei[2];Zhang, Wenjun[2];Yuan, Yuchen[1];Sun, Liyun[1,2];Fan, Jianhua[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Dept Appl Biol, Shanghai 200237, Peoples R China;[3]Shihezi Univ, Sch Chem & Chem Engn, State Key Lab Incubat Base Green Proc Chem Engn, Shihezi 832003, Peoples R China

年份：2025

卷号：433

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20252118479469);WOS:【SCI-EXPANDED(收录号:WOS:001499519000002)】；

基金：This work was sponsored by National Key Research and Development Project of China 2020YFA0907304, Ningbo "Innovation Yongjiang 2035" Key R&D Programme-International Sci-tech Cooperation Projects 2024H002, Shanghai Science and Technology Innovation Action Plan 24HC2820800, Natural Science Foundation of Shanghai 23ZR1415100 and 24ZR1490800, Funding Project of the State Key Laboratory of Bioreactor Engineering and Shanghai Collaborative Innovation Center for Biomanufacturing Technology.

语种：英文

外文关键词：Microalgae; Cultivation optimization; Carotenoid; Column photobioreactor; Antioxidant activity

摘要：As a promising marine bioresource, Amphidinium carterae represents a significant source of peridinin, a light-harvesting carotenoid exclusive to dinoflagellates. However, its low cultivation density has hindered its exploitation, particularly for efficient peridinin production. This study initially screened the optimal light intensity of 55 mu mol/m2/s and a mixotrophic culture condition supplemented with 5 g/L glycerol for A. carterae cultivation. Subsequently, a combined cultivation strategy achieved the highest cell density of 9.76 x 106 cells/ mL and a peridinin yield of 32.44 mg/L. Scale-up cultivation in column photobioreactors demonstrated the robust adaptability of A. carterae, as evidenced by the photosynthetic and respiratory rate measurements. Furthermore, comprehensive in vitro antioxidant assessments revealed the potent antioxidant activity of peridinin, which exhibited comparable efficacy to fucoxanthin and superior performance relative to R-carotene. Through systematic optimization, this study has achieved the highest cell density and peridinin yield in A. carterae reported thus far.