文献类型：期刊文献

英文题名：An electrochemical aptasensor based on multi-walled carbon nanotubes loaded with PtCu nanoparticles as signal label for ultrasensitive detection of adenosine

作者：Cao, Shida[1];Zhao, Hongli[1];Chen, Kaicha[1];Zhou, Fangfang[1];Lan, Minbo[1,2]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Res Ctr Anal & Test, Shanghai 200237, Peoples R China

年份：2023

卷号：1260

外文期刊名：ANALYTICA CHIMICA ACTA

收录：;EI(收录号：20231613900499);WOS:【SCI-EXPANDED(收录号:WOS:000984756700001)】；

基金：This work was supported by the Science and Technology Commission of Shanghai Municipality (STCSM, 21JM0010400) ; and the Funda- mental Research Funds for the Central Universities (JKJ01221718) .

语种：英文

外文关键词：Adenosine; PtCu nanoparticles; Multi-walled carbon nanotubes; Amperometric; Electrochemical aptasensor

摘要：Adenosine, as an endogenous nucleoside modulator, plays an important role in heart rate regulation, neuro-transmission, and control of the respiratory system and thus it is significantly important to realize its sensitive detection. Herein, a highly sensitive electrochemical aptasensor for adenosine detection was proposed by using multi-walled carbon nanotubes (MWCNTs) as support matrix loading PtCu nanoparticles (PtCu-MWCNTs) to amplify signal. On one hand, disposable screen-printing gold electrodes (SPGEs) were used as superb sensing base to ensure the stable connection of aptamers 1 (ssDNA1). On the other hand, the PtCu-MWCNTs complex was synthesized through a one-pot method, which not only can precisely control the proportion of metal mass in the product but also exhibited superior electrocatalytic activity towards H2O2. The recognition reactions were achieved by stepwise incubation of ssDNA1, ssDNA2-PtCu-MWCNTs (denoted as ssDNA2-label), and adenosine on the SPGEs. As a result, the constructed electrochemical aptasensor exhibited a wide linear range from 10 nM to 1.0 mu M with a low detection limit of 1.0 nM (S/N = 3) for adenosine detection. The aptasensor also suc-cessfully realized the adenosine detection in human serum samples, which means that the proposed aptasensor holds a potential application in point-of-care detection.