文献类型：期刊文献

英文题名：Engineering of Trichoderma reesei for enhanced degradation of lignocellulosic biomass by truncation of the cellulase activator ACE3

作者：Chen, Yumeng[1];Wu, Chuan[1];Fan, Xingjia[1];Zhao, Xinqing[2];Zhao, Xihua[3];Shen, Tao[4];Wei, Dongzhi[1];Wang, Wei[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, POB 311,130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, State Key Lab Microbial Metab, Joint Int Res Lab Metab & Dev Sci, Shanghai 200240, Peoples R China;[3]Jiangxi Normal Univ, Coll Life Sci, Nanchang 330022, Jiangxi, Peoples R China;[4]Sunson Ind Grp Co Ltd, Beijing, Peoples R China

年份：2020

卷号：13

期号：1

外文期刊名：BIOTECHNOLOGY FOR BIOFUELS

收录：;EI(收录号：20201608427507);WOS:【SCI-EXPANDED(收录号:WOS:000523745600002)】；

基金：The project funded by China Postdoctoral Science Foundation funded project (No. 2019M661402), the Open Funding Project of the State Key Laboratory of Bioreactor Engineering, and the Fundamental Research Funds for the Central Universities (No. 222201714053).

语种：英文

外文关键词：Trichoderma reesei; Genetic engineering; Truncated ACE3; Cellulase production; Lignocellulosic biomass

摘要：Background The filamentous fungus Trichoderma reesei is a major workhorse employed to produce cellulase, which hydrolyzes lignocellulosic biomass for the production of cellulosic ethanol and bio-based products. However, the economic efficiency of biorefineries is still low. Results In this study, the truncation of cellulase activator ACE3 was identified and characterized in T. reesei classical mutant NG14 and its direct descendants for the first time. We demonstrated that the truncated ACE3 is the crucial cause of cellulase hyper-production in T. reesei NG14 branch. Replacing the native ACE3 with truncated ACE3 in other T. reesei strains remarkably improves cellulase production. By truncating ACE3, we engineered a T. reesei mutant, PC-3-7-A723, capable of producing more cellulase than other strains. In a 30-L fermenter, fed-batch fermentation with PC-3-7-A723 drastically increased the maximum cellulase titer (FPase) to 102.63 IU/mL at 240 h, which constitutes a 20-30% improvement to that of the parental strain PC-3-7. Conclusions This work characterized the function of truncated ACE3 and demonstrated that analysis of classical mutants allows rational engineering of mutant strains with improved cellulase production necessary to process lignocellulosic biomass. Our rational engineering strategy might be useful for enhancing the production of other bio-based products.