文献类型：期刊文献

中文题名：大肠杆菌莽草酸生物合成途径的基因操作

英文题名：Genetic Manipulation of the Genes Related with Shikimate Metabolism of Escherichia coli

作者：杨捷[1];钱晋[1];叶江[1];吴海珍[1];张惠展[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2009

卷号：35

期号：2

起止页码：207

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：莽草酸;基因敲除;强化表达;发酵

外文关键词：shikimic acid; gene disruption; over-expression; fermentation

摘要：为了调查大肠杆菌中相关基因的遗传操作对莽草酸途径和莽草酸积累的影响,利用温度敏感型质粒敲除了大肠杆菌JM83染色体DNA上编码莽草酸激酶的aroL基因,获得了该基因缺陷株JDL02;同时从大肠杆菌JM83染色体中分别克隆了aroG、ppsA和tktA3个基因,构建了多个表达质粒导入JDL02,得到了一系列重组菌。摇瓶发酵显示aroG基因的作用最为明显,各重组菌莽草酸产量均有不同程度的提高;在等生物量情况下,JDL02/pTrc-aroG-tktA的莽草酸产量是JM83的18.35倍,其摇瓶产量为94.33 mg/L。
In order to investigate how the genetic manipulation with related genes in Escherichia coli impacts the shikimate metabolism and the accumulation of shikimic acid, shikimate kinase II gene （aroL） of Escherichia coli JM83 was disrupted using temperature sensitive plasmid and the mutant was named as JDL02. Meanwhile, ppsA, tktA and aroG gene were cloned from Escherichia coli JM83. A series of expression plasmids were constructed and transferred into JDL02. Flask fermentation verified that the yields of all the recombinant strains are improved. In the same bacterial density condition, the shikimic acid yield of JDL02/pTre-aroG-tktA strain was 18.35 times of the control JM83, and the flask fermenta- tion yield is 94.33 mg/L.