文献类型：会议论文

英文题名：Recovery of L-(+)-lactic acid by anion exchange resin Amberlite IRA-400

作者：Cao, XJ; Yun, HS; Koo, YM

机构：[1]Inha Univ, Dept Biol Engn, Inchon 402751, South Korea;[2]E China Univ Sci & Technol, Dept Biochem Engn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

会议论文集：7th Young Asian Biochemical Engineers Community Symposium

会议日期：OCT 28-30, 2001

会议地点：E CHINA UNIV SCI & TECHNOL, SHANGHAI, PEOPLES R CHINA

主办单位：E CHINA UNIV SCI & TECHNOL

语种：英文

外文关键词：adsorption; lactic acid; separation; anion ion exchange resin; IRA-400

摘要：Amberlite IRA-400 anion exchange resin was applied for the recovery of L-(+)-lactic acid from fermentation broth. Adsorption isotherm and breakthrough curves for the separation of L-(+)-lactic acid were obtained at pH 5.0 and 2.0, respectively. Different types of adsorption isotherms were found at pH above and below the pK(a) (3.86) of lactic acid. The isotherm was found to be a Langmuir type at pH 5.0, whereas the isotherm was type II (multilayer adsorption) at pH 2.0. At pH 5.0, the maximum adsorption capacity of the resin, q(m) and dissociation constant, K-d were 222.46 mg/g wet resin and 60.7 mg/ml, respectively. Breakthrough curve for the separation of lactic acid from fermentation broth was also obtained. The maximum adsorption capacity (197.09 mg/g wet resin) at pH 5.0 was much higher than that at pH 2.0 (106 mg/g wet resin). Proper elution and washing conditions were sought by using H2SO4, methanol, ammonia or their mixtures as eluant. When column separation was performed at pH 5.0 by using 50% (v/v) methanol as washing solvent and 1.0 M H2SO4 as eluant, the total yield was 86.21%. However, the total yield was 92.11% when the column separation was performed at pH 2.0 and water was used as eluant. (C) 2002 Elsevier Science B.V. All rights reserved.