文献类型：期刊文献

英文题名：Production of polymalic acid and malic acid from xylose and corncob hydrolysate by a novel Aureobasidium pullulans YJ 6-11 strain

作者：Zou, Xiang[1,2];Yang, Jing[1];Tian, Xu[1];Guo, Meijing[3];Li, Zhenghua[4];Li, Yunzheng[4]

机构：[1]Southwest Univ, Chongqing Engn Res Ctr Pharmaceut Proc & Qual Con, Coll Pharmaceut Sci, Chongqing 400715, Peoples R China;[2]Chongqing Univ, Key Lab Biorheol Sci & Technol, Minist Educ, Chongqing 400044, Peoples R China;[3]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[4]Anhui Sealong Biotechnol Co Ltd, Anhui Engn Res Mal Acid Technol, Bengbu 233316, Peoples R China

年份：2016

卷号：51

期号：1

起止页码：16

外文期刊名：PROCESS BIOCHEMISTRY

收录：;EI(收录号：20155201731361);WOS:【SCI-EXPANDED(收录号:WOS:000369202800003)】；

基金：This study was supported in part by grants from the National High Technology Research and Development Program of China (863 Program) (2015AA021005 and 2014AA021205), National Natural Science Foundation of China (Grant No. 31571816), Fundamental Research Funds for the Central Universities (XDJK2013B039 and 2362014XK07), and Visiting Scholar Foundation of Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, China (CQKLBST-2014-006).

语种：英文

外文关键词：Aureobasidium pullulans; Corncob hydrolysate; Fermentation; Malic acid; Polymalic acid; Xlyose

摘要：Polymalic acid (PMA) is a water-soluble biopolymer with many attractive properties for pharmaceutical applications. Its monomer, L-malic acid, is a potential C4 platform chemical, which is widely used in the food industry. In this study, a new strain with a high PMA yield from xylose was isolated and identified as Aureobasidium pullulans based on the morphological and phylogenetic analyzes. This strain produced PMA from xylose at a comparable or higher rate than glucose, and PMA produced from xylose had a similar molecular weight to that produced from glucose. A high final PMA titer of 80.4 g/L (91.2 g/L of malic acid after hydrolysis) was achieved using fed-batch fermentation with xylose in a 5-L fermentor at a PMA productivity of 0.52 g/L h. Corncob hydrolysate could be better utilized producing 28.6 g/L of PMA (or 32.4g/L malic acid) in batch fermentation. PMA was further characterized for its chemical structure. This strain can be used to produce PMA and malic acid from xylose and lignocellulosic biomass hydrolysates. (C) 2015 Elsevier Ltd. All rights reserved.