文献类型：期刊文献

英文题名：Evaluation of a DNA vaccine with self-designed CpG sequences against J genotype IHNV infection in rainbow trout (Oncorhynchus mykiss)

作者：Zhao, Li[1];Wu, Di[2];Wang, Jing[2];Wu, Kaixing[2];Ren, Yuhong[2,3,4];Liu, Qin[2,3,4];Bao, Endong[1];Shao, Shuai[2,3,4]

机构：[1]Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China;[4]Lab Aquat Anim Dis MOA, Shanghai 200237, Peoples R China

年份：2024

卷号：154

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001334367400001)】；

基金：This work was supported by grants from the National Key Research and Development Program (2023YFD2400702) , Qingdao Marine Science and Technology Center (11-04) , and Shanghai Agricultural Science and Technology Innovation Program (K2023014) .

语种：英文

外文关键词：CpG; DNA vaccine; J genotype; Infectious hematopoietic necrosis virus IHNV

摘要：Rainbow trout suffer from infectious hematopoietic necrosis virus (IHNV) outbreaks, which lead to massive mortality and huge economic loss worldwide. The approved commercial vaccine is used for the prevention of IHN in Canada. Given that Chinese domestic J-genotype isolates are different from North American IHNV isolates, the development of an effective DNA vaccine against Chinese J-genotype isolates is urgent. In this study, we developed a DNA vaccine encoding glycoprotein based on our previously isolated IHNV GS21 strain and selfdesigned CpG sequences were supplemented as molecular adjuvants. The vaccinated rainbow trout were significantly protected against IHNV with approximately a relative percent survival (RPS) of 94.74 % compared to the unvaccinated group. Moreover, the specific antibody of IgM and neutralizing antibody (NAb) was significantly provoked after the vaccination. Particularly, the antiviral immune response was rapidly evoked in the early stage of vaccination including the up-regulation of Mx-1, IFN-I, and IFN-gamma. The IHNV load in vaccinated fish was apparently lower than that in the unvaccinated group. Furthermore, the integration of exogenous genes into the host chromosome and the spread of antimicrobial-resistant genes were not found. These results suggested that our vaccine enhances robust immune responses and evokes considerable protection against IHNV with limited genetically modified risk, which is an effective and promising vaccine candidate for further prevention of IHNV outbreaks.