文献类型：期刊文献

英文题名：AIE-active luminogens as highly efficient free-radical ROS photogenerator for image-guided photodynamic therapy

作者：Liu, Zhenxing[1,2];Wang, Qi[1,2];Qiu, Wanshan[3];Lyu, Yanting[1,2];Zhu, Zhirong[1,2];Zhao, Xiaolei[1,2];Zhu, Wei-Hong[1,2]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Inst Fine Chem, Feringa Nobel Prize Scientist Joint Res Ctr,Joint, Sch Chem & Mol Engn,Frontiers Sci Ctr Materiobiol, Shanghai 200237, Peoples R China;[3]Fudan Univ, Dept Cardiothorac Surg, Childrens Hosp, Shanghai 201102, Peoples R China

年份：2022

卷号：13

期号：12

起止页码：3599

外文期刊名：CHEMICAL SCIENCE

收录：;EI(收录号：20221311858400);WOS:【SCI-EXPANDED(收录号:WOS:000765899600001)】；

基金：This work was supported by the NSFC Science Center Program (21788102), National Key Research and Development Program of China (2021YFA0910000), NSFC/China (91959202, 21974047 and 21622602), and Shanghai Municipal Science and Technology Major Project (2018SHZDZX03). All of the animal experimental procedures were performed according to the protocols approved by the ethics board of Ruijin Hospital.

语种：英文

外文关键词：Mammals - Free radicals - Oxygen - Charge transfer - Fluorescence - Hydrophilicity - Photodynamic therapy - Cancer cells - Infrared devices - Bacteria - Diseases

摘要：Image-guided photodynamic therapy (PDT) can realize highly precise and effective therapy via the integration of imaging and therapy, and has created high requirements for photosensitizers. However, the PDT modality usually utilizes conventional type II photosensitizers, resulting in unsatisfactory imaging and therapeutic outcomes due to aggregation-caused quenching (ACQ), "always on" fluorescence and strong oxygen dependence. Herein, we report the type I-based aggregation-induced emission (AIE) photosensitizer TCM-CPS with low oxygen dependence, near-infrared (NIR) emission and "off-on" fluorescence; in particular, it produces more reactive oxygen species (ROS) than commercially available Chlorin e6 and Rose Bengal. In the rational design of the AIE-based photosensitizer TCM-CPS, the strongly electron-donating carbazole unit and pi-thiophene bridge distinctly extend the emission wavelength and decrease the autofluorescence interference in bio-imaging, and the hydrophilic pyridinium salt group guarantees good molecular dispersion and maintains the fluorescence-off state in the aqueous system to decrease the initial fluorescence background. Moreover, the strong donor-pi-acceptor (D-pi-A) character in TCM-CPS greatly separates the HOMO-LUMO distribution, enhancing the ROS generation, and TCM-CPS was constructed as a type I photosensitizer with the assistance of strong intramolecular charge transfer in the electron-rich anion-pi(+) structure. Based on its favorable hydrophilicity and photosensitivity, TCM-CPS was found to be a highly efficient free-radical ROS photogenerator for both visualizing cells using light-up NIR fluorescence and efficiently killing cancer cells upon light irradiation. The positively charged TCM-CPS could quickly bind to bacteria via electrostatic interactions to provide a light-up signal and kill bacteria at a low concentration. In the PDT treatment of bacteria-infected mice, the mice exhibited accelerated wound healing with low wound infection. Thus, the AIE-based type I photosensitizer TCM-CPS has great potential to replace commercially available photosensitizers in the image-guided PDT modality for the treatment of cancer and bacterial infection.