文献类型：期刊文献

中文题名：纳米磁粉固定化酶催化合成α-D-葡萄糖-1-磷酸(英文)

英文题名：Efficient Synthesis of α-D-Glucose-1-Phosphate by Maltodextrin Phosphorylase Immobilized on Amino-functionalized Magnetic Nanoparticles

作者：董青[1];欧阳立明[1];刘建文[1];许建和[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2010

卷号：31

期号：10

起止页码：1227

中文期刊名：催化学报

外文期刊名：CHINESE JOURNAL OF CATALYSIS

收录：CSTPCD;;EI(收录号：20104313334079);Scopus;WOS:【SCI-EXPANDED(收录号:WOS:000284388000007)】；北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：Supported by the National Natural Science Foundation of China (20773038, 20902023), the National Basic Research Program of China (973 Program, 2009CB724706, 2009ZX09501-016), the China National Special Fund for State Key Laboratory of Bioreactor Engineering (2060204), and the Shanghai Leading Academic Discipline Project (B505).

语种：中文

中文关键词：α-D-葡萄糖-1-磷酸;麦芽糊精磷酸化酶;麦芽糊精;磁性纳米颗粒;固定化生物催化剂

外文关键词：α-D-Glucose-1-phosphate; maltodextrin phosphorylase; maltodextrin; magnetic nanoparticle; immobilized biocatalyst

摘要：建立了以麦芽糊精和磷酸盐为底物,在常温下合成α-D-葡萄糖-1-磷酸的生物催化体系.从大肠杆菌K12中克隆表达了麦芽糊精磷酸化酶,并固定化在氨基修饰的磁性纳米颗粒上,以便于酶的回收和重复利用.在优化的反应条件下,于200ml体系中连续使用该固定化酶8批次,催化合成了α-D-葡萄糖-1-磷酸.经过简单的纯化步骤,最终得到440mg产品,分离产率为70.5%.
α-D-Glucose-1-phosphate （Glc-1-P） is an expensive intermediate in the biosynthesis of nucleotide glucose. This paper describes a biocatalytic system for the efficient synthesis of Glc-1-P from the low cost raw materials： maltodextrin and phosphate at ordinary tempera-tures. After molecular cloning and the expression of a maltodextrin phosphorylase （MalPase） gene from E. coli （Escherichia coli） K12, the resultant recombinant enzyme was immobilized on amino-functionalized magnetic nanoparticles for recycling and repeated use. Conditions for the biocatalytic reaction were optimized and the immobilized MalPase could be easily recovered and reused over eight cycles in the repeated synthesis of Glc-1-P. After simple purification steps approximately 440 mg of crude product was obtained with a moderate isolation yield of 70.5%.