文献类型：期刊文献

英文题名：Chemical Fingerprint and Quantitative Analysis of Cirsium setosum by LC

作者：Lu, Yanhua[1];Song, Wei[1];Liang, Xinhua[1];Wei, Dongzhi[1];Zhou, Xiaoli[2]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Inst Technol, Sch Perfume & Aroma Technol, Shanghai 200235, Peoples R China

年份：2009

卷号：70

期号：1-2

起止页码：125

外文期刊名：CHROMATOGRAPHIA

收录：;EI(收录号：20242616364399);WOS:【SCI-EXPANDED(收录号:WOS:000268055600017)】；

基金：This work is supported by Shanghai Leading Academic Discipline Project (B505).

语种：英文

外文关键词：Column liquid chromatography; Fingerprint analysis; Phenolic compounds; Cirsium setosum

摘要：A reverse phase liquid chromatography method with diode array detection was developed to evaluate the quality of Cirsium setosum through establishing chromatographic fingerprint and simultaneous determination of six phenolic compounds, namely chlorogenic acid, caffeic acid, rutin, linarin, luteolin and apigenin. The chromatographic separation was performed on an Agilent SB-C(18) column (250 x 4.6 mm, 5.0 mu m) with a gradient elution program using a mixture of acetonitrile and 0.5% aqueous acetic acid (v/v) as mobile phase within 25 min at 326 nm wavelength. The correlation coefficients of similarity were determined from the LC fingerprints, and they shared a close similarity. The LC with electrospray ionization mass spectrometry experiment was performed to further confirm the identity of phenolic compounds. The six phenolic compounds showed good regression (R (2) > 0.9995) within test ranges and the recovery of the method was in the range of 95.8-102.8%. In addition, the content of those six phenolic compounds in C. setosum growing in different locations of China was determined to establish the effectiveness of the method. The results indicated that the developed method by having a combination of chromatographic fingerprint and quantification analysis could be readily utilized as a quality control method for C. setosum and its related traditional Chinese medicinal preparations.