文献类型：期刊文献

中文题名：基于液质联用技术解析和降低螺旋霉素发酵杂质

英文题名：LC-ESI/MS Identification and Reduction of Impurities in Spiramycin Fermentation

作者：李友元[1];陈长华[1];顾熠峰[1];李晓勇[2]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237;[2]河南天方药业股份有限公司,河南463003

年份：2005

卷号：21

期号：2

起止页码：275

中文期刊名：生物工程学报

外文期刊名：Chinese Journal of Biotechnology

收录：MEDLINE(收录号：16013489);CSTPCD;;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；PubMed;

语种：中文

中文关键词：液质联用;螺旋霉素;杂质;优化

外文关键词：LC-ESI/MS, spiramycin, impurity, optimization

摘要：目前螺旋霉素生物合成的发酵杂质含量过高 ,导致成品收率过低 ,杂质含量不符合药典规定。为解决这一问题 ,首先利用LC -MS联用技术定性分析了 4种螺旋霉素发酵杂质的结构 ,结合螺旋霉素生物合成途径分析 ,它们都起因于螺旋霉素生物合成糖基化过程的紊乱。氮源尤其是铵盐对螺旋霉素生物合成糖基化过程有重要的调节作用。进一步的氮源优化使螺旋霉素发酵液中 4种杂质含量降低 2 2 %～ 88% ,杂质含量全部低于欧洲药典标准。
There are several impurities in the spiramycin fermentation broth whic h leads to a lower yield and lower quality of the product. Four impurities in sp iramycin broth have been simultaneously separated and identified by LC-ESI/MS. T he generation of these impurities was attributed to the fluctuation of glucosyla tion in spiramycin biosynthesis. Nitrogen sources, ammonium in particular, were found to play an important role at the glucosylation. Aided with the information of LC-ESI/MS analysis and subsequent optimization of the culture medium, bette r culture medium of shake flask was designed, which leads to reduction of impuri ties by 22%～88%.