文献类型：期刊文献

英文题名：Antitoxin HigA orchestrates T3SS/T6SS expression in aquatic pathogen Edwardsiella piscicida

作者：Li, Chunli[1];Tian, Yufeng[1];Wang, Qiyao[1,2,3];Shao, Shuai[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]Lab Aquat Anim Dis MARA, Shanghai, Peoples R China;[3]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai, Peoples R China

年份：2025

卷号：300

外文期刊名：MICROBIOLOGICAL RESEARCH

收录：;EI(收录号：20252918808590);WOS:【SCI-EXPANDED(收录号:WOS:001535197700001)】；

基金：We thank Miss YH. Liu for her technical support. This work was supported by grants from the National Natural Science Foundation of China (32373183) and the China Agriculture Research System of MOF and MARA (CARS-47) .

语种：英文

外文关键词：Edwardsiella piscicida; Toxin-antitoxin systems; HigA; T3SS; T6SS

摘要：Toxin-antitoxin (TA) systems are widely distributed in bacteria and archaea, playing crucial roles in multiple biological processes. As the type II TA system in Edwardsiella piscicida, HigB contributes to biofilm formation, intracellular propagation, and host infection. Here, we demonstrated that the cognate antitoxin HigA functions as a key virulence transcriptional regulator. RNA-seq and GSEA analysis revealed that HigA orchestrates the expression of genes associated with type III and type VI secretion systems. Extracellular protein profiles further confirmed that HigA promotes the secretion of T3SS/T6SS proteins. In addition, HigA enhances the pathogenesis of E. piscicida in zebrafish. Collectively, our results highlight that HigA functions as a transcriptional activator, integrating TA system regulation into the broader virulence network in E. piscicida.