文献类型：期刊文献

英文题名：Improvement of a dry formulation ofPseudomonas protegensSN15-2 againstRalstonia solanacearumby combination of hyperosmotic cultivation with fluidized-bed drying

作者：Wang, Xiaobing[1];Tang, Danyan[1];Wang, Wei[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2020

卷号：65

期号：6

起止页码：751

外文期刊名：BIOCONTROL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000558631200001)】；

基金：The authors gratefully acknowledge financial support from the National Key Research and Development Program of China (Nos. 2017YFD0200400; 2017YFD0201100) and the Science and Technology Commission of Shanghai Municipality, China (No. 18391902500).

语种：英文

外文关键词：Pseudomonas protegens; Hyperosmotic cultivation; Fluidized-bed drying; Biocontrol

摘要：The application value ofPseudomonas protegensin agriculture has been widely studied, but the manufacturing processes used to produceP.protegenslimits its usefulness as a biological control agent because of its poor shelf life due to low stress resistance during processing. We combined hyperosmotic cultivation and fluidized-bed drying to prepare much more stableP.protegenspreparations. During drying and cryogenic storage, the viability of hyperosmotic cells was significantly improved compared to that of cells prepared by previous methods. Addition of skim milk provided an excellent protective effect for both hyperosmotic and standard cells. In two field trials, when the concentration of theP.protegenspreparation was 0.333% w/v, the biocontrol efficacy against tomato bacterial wilt was more than 80%, which was significantly higher than that of the control provided by the antibiotics zhongshengmycin and streptomycin sulfate. The results show thatP.protegensproducts can be successfully prepared by combination of hyperosmotic cultivation and fluidized-bed drying.