文献类型：期刊文献

英文题名：A versatile graphene-based fluorescence "on/off" switch for multiplex detection of various targets

作者：Zhang, Min[1];Yin, Bin-Cheng[1];Tan, Weihong[2,3];Ye, Bang-Ce[1]

机构：[1]E China Univ Sci & Technol, Lab Biosyst & Microanal, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Univ Florida, Dept Chem, Ctr Res Bio Nano Interface, Gainesville, FL 32611 USA;[3]Univ Florida, Shands Canc Ctr, Gainesville, FL 32611 USA

年份：2011

卷号：26

期号：7

起止页码：3260

外文期刊名：BIOSENSORS & BIOELECTRONICS

收录：;EI(收录号：20110813685656);WOS:【SCI-EXPANDED(收录号:WOS:000288730700026)】；

基金：This work was financially supported by NSF (21075040), the Shanghai Project (09JC1404100), the SKLBE (2060204), Program for New Century Excellent Talents (NCET-07-0287), and the Fundamental Research Funds for the Central Universities. We are grateful to Prof. Yu Chen for kindly providing graphene oxide.

语种：英文

外文关键词：Graphene oxide; Quencher; Fluorescence; Multiplexed detection; Molecular beacon

摘要：We have designed a versatile molecular beacon (MB)-like probe for the multiplex sensing of targets such as sequence-specific DNA, protein, metal ions and small molecule compounds based on the self-assembled ssDNA-graphene oxide (ssDNA-GO) architecture. The probe employs fluorescence "on/off' switching strategy in a single step in homogeneous solution. Compared to traditional molecular beacons, the proposed design is simple to prepare and manipulate and has little background interference, but still gives superior sensitivity and rapid response. More importantly, this ssDNA-GO architecture can serve as a universal beacon platform by simply changing the types of ssDNA sequences for the different targets. In this work, the ssDNA-GO architecture probe has been successfully applied in the multiplex detection of sequence-specific DNA, thrombin, Ag+, Hg2+ and cysteine, and the limit of detection was 1 nM, 5 nM, 20 nM, 5.7 nM and 60 nM, respectively. The results demonstrate that the ssDNA-GO architecture can be an excellent and versatile platform for sensing multiplex analytes, easily replacing the universal molecular beacon. (C) 2011 Elsevier B.V. All rights reserved.