文献类型：期刊文献

中文题名：壳聚糖絮凝法精制合欢皮多糖水提液的工艺

英文题名：Flocculating purification process of total polysaccharides from bark of Albizzia julibrissin by using chitosan

作者：韩乐[1];孙晓海[1];韩伟[1]

机构：[1]华东理工大学中药现代化工程中心,上海200237

年份：2014

卷号：12

期号：2

起止页码：38

中文期刊名：生物加工过程

外文期刊名：Chinese Journal of Bioprocess Engineering

收录：CSTPCD;;CSCD:【CSCD_E2013_2014】；

基金：国家大学生创新计划(111025124)

语种：中文

中文关键词：合欢皮;壳聚糖;絮凝;多糖

外文关键词：Albizzia julibrissin;chitosan;flocculating;polysaccharide;Albizzia julibrissin;chitosan;flocculating;polysaccharide

摘要：采用壳聚糖对合欢皮总多糖的絮凝纯化工艺进行研究。通过单因素和正交试验,得到优化的工艺条件:壳聚糖用量为生药量0.8 mL/g,絮凝温度35℃,絮凝时间4 h,药液浓缩比(质量体积比)为1∶10(g/mL),在此条件下,多糖保留率、脱蛋白率和多糖纯度分别为91.23%、22.26%和47.27%,优于传统的水提醇沉法。
Through single factor and orthogonal experimental designs, the optimun conditions were obtained as follows：the dosage of chitosan was 0.8 mL/g, temperature 35 ℃, flocculation time 4 h, determined as 1∶10 of drug density.Under the optimum conditions,polysaccharide retention rate,protein removal rate,and the purity of polysaccharide were obtained as follows：91.23%,22.26% and 47.27%, respectively.The flocculating purification of total polysaccharides was superior to the traditional method of extracting with water and depositing with alcohol.