文献类型：期刊文献

英文题名：Spatiotemporal Imaging of Cellular Energy Metabolism with Genetically-Encoded Fluorescent Sensors in Brain

作者：Zhang, Zhuo[1,3];Chen, Weicai[1,3];Zhao, Yuzheng[1,3];Yang, Yi[1,2]

机构：[1]East China Univ Sci & Technol, Shanghai Collaborat Innovat Ctr Biomfg Technol, State Key Lab Bioreactor Engn, Synthet Biol & Biotechnol Lab, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Shanghai Inst Biol Sci, CAS Ctr Excellence Brain Sci, Optogenet & Synthet Biol Interdisciplinary Res Ct, Shanghai 200031, Peoples R China;[3]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China

年份：2018

卷号：34

期号：5

起止页码：875

外文期刊名：NEUROSCIENCE BULLETIN

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000444005200021)】；

基金：This review was supported by the National Key Research and Development Program of China (2017YFA050400 and 2017YFC0906900), the National Natural Science Foundation of China (31722033, 91649123, 31671484, 31225008, and 31470833), the Shanghai Science and Technology Commission (14XD1401400, 16430723100, and 15YF1402600), Young Elite Scientists Sponsorship Program by China Association for Science and Technology (to YZ), Shanghai Young Top-notch Talent (to YZ), the State Key Laboratory of Bioreactor Engineering (to YY), and Fundamental Research Funds for the Central Universities (to YY and YZ).

语种：英文

外文关键词：Energy metabolism; Astrocyte; Neuron; Genetically encoded fluorescent sensor; Real time monitoring

摘要：The brain has very high energy requirements and consumes 20% of the oxygen and 25% of the glucose in the human body. Therefore, the molecular mechanism underlying how the brain metabolizes substances to support neural activity is a fundamental issue for neuroscience studies. A well-known model in the brain, the astrocyte-neuron lactate shuttle, postulates that glucose uptake and glycolytic activity are enhanced in astrocytes upon neuronal activation and that astrocytes transport lactate into neurons to fulfill their energy requirements. Current evidence for this hypothesis has yet to reach a clear consensus, and new concepts beyond the shuttle hypothesis are emerging. The discrepancy is largely attributed to the lack of a critical method for real-time monitoring of metabolic dynamics at cellular resolution. Recent advances in fluorescent protein-based sensors allow the generation of a sensitive, specific, real-time readout of subcellular metabolites and fill the current technological gap. Here, we summarize the development of genetically encoded metabolite sensors and their applications in assessing cell metabolism in living cells and in vivo, and we believe that these tools will help to address the issue of elucidating neural energy metabolism.