文献类型：期刊文献

英文题名：Skin-Soothing Properties of Juniperus formosana Hayata Revealed by Bioactivity-Based Molecular Networking

作者：Han, Qi-Feng[1];Wu, Meng-Qi[1];Zhu, Mu-Lan[2];Liu, Yu-Xuan[1];Yang, Xiang[1];Zhang, Wen-Qing[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai, Peoples R China;[2]CAS Ctr Excellence Mol Plant Sci, Natl Key Lab Plant Mol Genet NKLPMG, Shanghai, Peoples R China

年份：2026

卷号：23

期号：2

外文期刊名：CHEMISTRY & BIODIVERSITY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001634487600001)】；

语种：英文

外文关键词：Juniperus formosana Hayata; MCP-1; molecular networking; skin soothing; TRPA1

摘要：Juniperus formosana Hayata, a Tibetan medicinal plant, has been used for over a millennium in China to treat dermatitis and itching, However, its application in cosmetic and personal care products has been limited due to a lack of scientific evidence. This study aimed to identify its active compounds and biological mechanism. Guided by bioactivity-based molecular networking, a skin-soothing active fraction, predominantly composed of the theaflavin analog (84.47%), was purified from J. formosana extract. This fraction significantly inhibited the secretion and mRNA expression of the chemokine MCP-1 in inflammatory keratinocytes, suppressed inflammation-induced monocyte recruitment and its activation marker CD86. In addition, it alleviated inflammation-induced desensitization damage of TRPA1, an ion channel associated with pain and itch. Molecular docking revealed that the theaflavin analog exhibits high binding affinity to both monomeric (-8.1 kcal/mol) and dimeric (-8.6 kcal/mol) forms of MCP-1, positioning it as a promising active compound for preventing skin inflammation J. formosana Hayata * molecular networking * skin soothing * MCP-1 * TRPA1exacerbation by interfering with the MCP-1/CCR2 axis.