文献类型：期刊文献

英文题名：Identification of the di-n-butyl phthalate-biodegrading strains and the biodegradation pathway in strain LMB-1

作者：Fang, Y.[1];Zhang, L. -S.[1];Wang, J.[1];Zhou, Y.[1];Ye, B. -C.[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Meilong RD 130, Shanghai 200237, Peoples R China;[2]Shihezi Univ, Sch Chem & Chem Engn, Xinjiang 832000, Peoples R China

年份：2017

卷号：53

期号：3

起止页码：310

外文期刊名：APPLIED BIOCHEMISTRY AND MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000401449900005)】；

基金：This research was supported by National Science Foundation of China (31401592).

语种：英文

外文关键词：phthalate; biodiversity; biodegradation; pathway; greenhouse soil

摘要：DNA isolated from a greenhouse soil (Nanjing, Jiangsu Province, China) was suitable for PCR amplification of gene segment coding for the 16S rRNA. Diverse PCR products were characterized by cloning and sequencing, and analysis of bacterial colonies showed the presence over 26 phyla. The most bacteria belonged to Proteobacteria, Actinobacteria, Gemmatimonadetes, Acidobacteria and Planctomycetes. Furthermore, after the enrichment procedure of DBP-degrading microorganisms, 4 strains were isolated from the soil sample with di-n-butyl phthalate (DBP) biodegradability, and they were identified to be Rhizobium sp., Streptomyces sp., Pseudomonas sp. and Acinetobacter sp. Analysis of the degradation products by LC-MS led to identification of metabolites of DBP in strain LMB-1 (identified as Rhizobium sp.) which suggests that DBP was degraded through beta-oxidation, demethylation, de-esterification and cleavage of aromatic ring.