文献类型：期刊文献

英文题名：Engineering wild-type robust Pediococcus acidilactici strain for high titer L- and D-lactic acid production from corn stover feedstock

作者：Yi, Xia[1];Zhang, Peng[1];Sun, Jiaoe[1];Tu, Yi[1];Gao, Qiuqiang[1];Zhang, Jian[1];Bao, Jie[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2016

卷号：217

起止页码：112

外文期刊名：JOURNAL OF BIOTECHNOLOGY

收录：;EI(收录号：20155001663181);WOS:【SCI-EXPANDED(收录号:WOS:000366757900016)】；

基金：This research was supported by the Natural Science Foundation of China (21306048, 31300070), the National High-Tech Program of China (2012AA022301, 2014AA021901) and the Fundamental Research Funds for the Central Universities of China (WF1314036).

语种：英文

外文关键词：Pediococcus acidilactici; L-Lactic acid; D-Lactic acid; Lignocellulose; Inhibitor tolerance; Biodetoxification

摘要：Pediococcus acidilactici TY112 producing L-lactic acid and P. acidilactici ZP26 producing ID-lactic acid, were engineered from the wild-type P. acidilactici DQ2 by ldhD or ldh gene disruption, and the robustness of the wild-type strain to the inhibitors derived from lignocellulose pretreatment was maintained well. In simultaneous saccharification and fermentation (SSF), 77.66 g L-1 of L-lactic acid and 76.76 g L-1 of D-lactic acid were obtained at 25% (w/w) solids content of dry dilute acid pretreated and biodetoxified corn stover feedstock. L- and D-Lactic acid yield and productivity were highly dependent on the inhibitor removal extent due to the significant down-regulation on the expressions of ldh and ldhD encoding lactate dehydrogenase by inhibitor, especially syringaldehyde and vanillin at the low concentrations. This study provided a prototype of industrial process for high titer L- and D-lactic acid production from lignocellulose feedstock. (C) 2015 Elsevier B.V. All rights reserved.