文献类型：期刊文献

中文题名：种球溶胀法制备有机聚合物色谱填料及其在纯化红景天苷中的应用

英文题名：Preparation of Organic Polymer Chromatographic Packings by Swelling Seeds and Their Application for Seperation of Salidroside

作者：黄海松[1];徐环昕[1];刘坐镇[1];陈骏[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2007

卷号：33

期号：4

起止页码：485

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：分散聚合;正丁醇;种球溶胀;色谱填料;红景天苷

外文关键词：dispersion polymerization; n-butanol; seeds swelling; chromatographic packings; salidroside

摘要：采用分散聚合法,以正丁醇为介质制成单分散的聚苯乙烯微球,应用该微球通过种球溶胀法制备聚苯乙烯-二乙烯苯(PSt-DVB)色谱填料。应用高压匀质机制备的活化种球和溶胀活化种球的乳液更稳定,液滴更小。该填料单分散性能优越,填装4.6 mm×150 mm的色谱柱背压低,可用作分析色谱和制备色谱。该色谱柱用于红景天苷的分离,可获得98%以上的纯品。
Monodisperse polystyrene particles as the swollen seeds for synthesizing macro-porous poly（styrene-co-divinylbenzene） organic polymer chromatographic packings were prepared by dispersion polymerization in n-butanol reaction media. And the stable fine emulsions for swelling seeds could be obtained by using a high-pressure homogenizer. The chromatographic packings exhibit excellent monodispersibility and low back pressure while packed into chromatographic column, and can be employed for the separation and purification of small biomolecules in analytical and preparative chromatography. Application of the chromatographic packings to the purification of salidroside by using ethanol-water as binary mobile phase, the purity of salidroside over 98% could be obtained.