文献类型：期刊文献

英文题名：Comparison between the effects of normoxia and hypoxia on antioxidant enzymes and glutathione redox state in ex vivo culture of CD34⁺ cells

作者：Fan, Jinli[1];Cai, Haibo[1];Yang, Shi[1];Yan, Lingli[1];Tan, WenSong[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2008

卷号：151

期号：2

起止页码：153

外文期刊名：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000259750400003)】；

基金：This research work was supported by the National Natural Science Foundation of China (20776043) and the Key Project Of Medicine, Shanghai (074319109).

语种：英文

外文关键词：CD34+hematopoietic stem and progenitor cells (HSPCs); glutathione; hypoxia; normoxia; oxidative stress

摘要：Hypoxia maintained biological characteristics of CD34(+) cells through keeping lower intracellular reactive oxygen specials (ROS) levels. The effects of normoxia and hypoxia on antioxidant enzymes and glutathione redox state were compared in this study. Hypoxia decreased the mRNA expression of both catalase (CAT) and glutathione peroxidase (GPX), but not affected mRNAs expression of superoxide dismutase (SOD). While the cellular GPX activities under hypoxia were apparently less than those under normoxia, neither SOD activities nor CAT activities were affected by hypoxia. The analysis of glutathione redox status and ROS products showed the lower oxidized glutathione (GSSG) levels, the higher reduced glutathione (GSH) levels, the higher GSH/GSSG ratios, and the less O-2- and H2O2 generation under hypoxia (versus normoxia). Meanwhile More primary CD34(+)CD38(-) cells were obtained when cultivation was performed under hypoxia or with N-acetyl cysteine (the precursor of GSH) under normoxia. These results demonstrated the different responses of anti-oxidative mechanism between normoxia and hypoxia. Additionally, the present study suggested that the GSH-GPX antioxidant system played an important role in HSPCs preservation by reducing peroxidation. (c) 2008 Elsevier Inc. All rights reserved.