文献类型：期刊文献

英文题名：Sustainable biosynthesis of curdlan from orange waste by using Alcaligenes faecalis: A systematically modeled approach

作者：Mohsin, Ali[1];Sun, Jingyun[1];Khan, Imran Mahmood[3];Hang, Haifeng[1];Tariq, Muhammad[2];Tian, Xiwei[1];Ahmed, Waqas[1];Niazi, Sobia[3];Zhuang, Yingping[1];Chu, Ju[1];Mohsin, Muhammad Zubair[5];Salim-ur-Rehman[4];Guo, Meijin[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Environm Engn, Shanghai 200237, Peoples R China;[3]Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Technol, Wuxi, Jiangsu, Peoples R China;[4]Univ Agr Faisalabad, Natl Inst Food Sci & Technol, Faisalabad 38040, Pakistan;[5]Univ Agr Faisalabad, Ctr Agr Biochem & Biotechnol, Faisalabad 38040, Pakistan

年份：2019

卷号：205

起止页码：626

外文期刊名：CARBOHYDRATE POLYMERS

收录：;EI(收录号：20184506043010);WOS:【SCI-EXPANDED(收录号:WOS:000450093200071)】；

基金：This work was financially supported by the National key research and development program 2017YFF0204602 and 2017ZX07402003.

语种：英文

外文关键词：Sustainable biosynthesis; Orange peels; Kinetics; Curdlan; Batch culture fermentation

摘要：This study presents an engineered approach for sustainable biosynthesis of curdlan by Alcaligenes faecalis using orange peels. To confirm the substrate suitability a four step study was organized. Firstly, drying of substrate was carried within temperature range of 60-120 degrees C, along with the application of moisture diffusion control model. Secondly, fermentation medium was obtained via saccharification and detoxification, releasing highest sugar at 72.34 g/L with phenolics removal of 95-98%. Thirdly, curdlan fermentation was conducted in detoxified orange peel hydrolysate followed by optimization of batch culture fermentation via kinetic modeling using Logistic and Luedeking-Piret equations. In 5 L bioreactor, highest specific growth rate (mu(m) = 0.233/h), highest curdlan production (P-m = 23.24 g/L) and growth associated rate constant (alpha = 3.403) were achieved. Moreover, the total sugar consumption and conversion rates were 83.27% and 53.20%. Lastly, characterization techniques such as FTIR, NMR, XRD, TGA, HPGPC and EDS were applied to biosynthesized curdlan for qualitative validation.