文献类型：期刊文献

英文题名：Harnessing α-L-fucosidase for in vivo cellular senescence imaging

作者：Koo, Seyoung[1];Won, Miae[1];Li, Hao[2];Kim, Won Young[1];Li, Mingle[1];Yan, Chenxu[3];Sharma, Amit[4];Guo, Zhiqian[3];Zhu, Wei-Hong[3];Sessler, Jonathan L.[5];Lee, Jin Yong[2];Kim, Jong Seung[1]

机构：[1]Korea Univ, Dept Chem, Seoul 02841, South Korea;[2]Sungkyunkwan Univ, Dept Chem, Suwon 16419, South Korea;[3]East China Univ Sci & Technol, Inst Fine Chem, Shanghai 200237, Peoples R China;[4]CSIR Cent Sci Instruments Org, Sect 30C, Chandigarh 160030, India;[5]Univ Texas Austin, Dept Chem, Austin, TX 78712 USA

年份：2021

卷号：12

期号：29

起止页码：10054

外文期刊名：CHEMICAL SCIENCE

收录：;EI(收录号：20213110712777);WOS:【SCI-EXPANDED(收录号:WOS:000668683700001)】；

基金：This work was supported by the National Research Foundation of Korea (CRI project no. 2018R1A3B1052702, 2019M3E5D1A01068998, J. S. K. and Brain Pool Program Grant No. 2020H1D3A1A02080172, M. L.), Basic Science Research Program (2020R1A6A3A01100551, M. W. and 2020R1A6A3A01100558, S. K., and 2019R1A6A1A10073079, J. Y. L). We are thankful for National Research Foundation of China (21788102) and a Department of Biotechnology, New Delhi (Ramalingaswami Fellowship 2019, Grant No. BT/RLF/Re-entry/59/2018, A. S.). Support from the Robert A. Welch Foundation (F-0018 to J. L. S.) is acknowledged. We also express appreciation to O. S. Shin (Korea University Guro Hospital) for a gift of the human dermal fibroblast (HDF) cell line.

语种：英文

外文关键词：Probes

摘要：Precise detection of cellular senescence may allow its role in biological systems to be evaluated more effectively, while supporting studies of therapeutic candidates designed to evade its detrimental effect on physical function. We report here studies of alpha-l-fucosidase (alpha-fuc) as a biomarker for cellular senescence and the development of an alpha-fuc-responsive aggregation induced emission (AIE) probe, termed QM-NH alpha fuc designed to complement more conventional probes based on beta-galactosidase (beta-gal). Using QM-NH alpha fuc, the onset of replicative-, reactive oxygen species (ROS)-, ultraviolet A (UVA)-, and drug-induced senescence could be probed effectively. QM-NH alpha fuc also proved capable of identifying senescent cells lacking beta-gal expression. The non-invasive real-time senescence tracking provided by QM-NH alpha fuc was validated in an in vivo senescence model. The results presented in this study lead us to suggest that the QM-NH alpha fuc could emerge as a useful tool for investigating senescence processes in biological systems.