文献类型：期刊文献

中文题名：毕赤氏酵母工程菌高密度发酵表达恶性疟原虫融合抗原的研究

英文题名：Expression of Chimeric Protein of Plasmodium falciparum in Yeast Recombinant Pichia pastoris in Cell-high-density Fermentation

作者：郭美锦[1];吴康华[1];储炬[1];庄英萍[1];张嗣良[1];黄大庆[2];张青锋[2];潘卫庆[2]

机构：[1]华东理工大学生物反应器工程国家重点实验室;[2]第二军医大学病原生物学教研室,上海200433

年份：2001

卷号：17

期号：4

起止页码：456

中文期刊名：生物工程学报

外文期刊名：Chinese Journal of Biotechnology

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；PubMed;

基金：国家"九五"863计划资助项目! (10 2 0 7 0 4 0 4);国家自然科学基金资助项目!(2 99760 13 )&&

语种：中文

中文关键词：甲醇营养型毕赤酵母;恶性疟原虫融合抗原;发酵条件;优化;高密度发酵;表达

外文关键词：methylotrophic Pichia pastoris, chimeric protein of Plasmodium falciparum, optimization of cultural conditions, high-density fermentation

摘要：The effects of cultural conditions,which were fermentation period when methanol was as sole carbon source,methanol concentration,range of pH,on the expression of the chimeric protein of Plasmodium falciparum in genetically engineered methylotrophic Pichia pastoris were investigated by shake flask experiments in this paper.The results showed:(1)fermentation period with methanol inducement is about 96 hours;(2)the optimum methanol concentration as sole carbon source is 10g/L;(3)the range of pH is from 6.0 to 7.0. On the base of above experimental results the cell high-density fermentation had been done on the FMG-5L fermentor with multi-sensors.The results showed that the cell optical density (OD 600) can reached 550 and the ma-ximum expression level of target proteins was 780mg/L which was 4 times higher or more than the shake flask culture’s.
The effects of cultural conditions,which were fermentation period when methanol was as sole carbon source,methanol concentration,range of pH,on the expression of the chimeric protein of Plasmodium falciparum in genetically engineered methylotrophic Pichia pastoris were investigated by shake flask experiments in this paper.The results showed:(1)fermentation period with methanol inducement is about 96 hours;(2)the optimum methanol concentration as sole carbon source is 10g/L;(3)the range of pH is from 6.0 to 7.0. On the base of above experimental results the cell high-density fermentation had been done on the FMG-5L fermentor with multi-sensors.The results showed that the cell optical density (OD 600) can reached 550 and the ma-ximum expression level of target proteins was 780mg/L which was 4 times higher or more than the shake flask culture's.