文献类型：期刊文献

英文题名：Enhanced L-ornithine production by systematic manipulation of L-ornithine metabolism in engineered Corynebacterium glutamicum S9114

作者：Zhang, Bin[1];Ren, Li-Quan[1];Yu, Miao[1];Zhou, Ying[1];Ye, Bang-Ce[1,2]

机构：[1]East China Univ Sci & Technol, Lab Biosyst & Microanal, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Zhejiang Univ Technol, Collaborat Innovat Ctr Yangtze River Delta Reg Gr, Coll Pharmaceut Sci, Hangzhou 310014, Zhejiang, Peoples R China

年份：2018

卷号：250

起止页码：60

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20174704415379);WOS:【SCI-EXPANDED(收录号:WOS:000430740000008)】；

基金：This work was supported by grants from the National Natural Science Foundation of China [grant numbers, 31730004 and 21575089].

语种：英文

外文关键词：Corynebacterium glutamicum; L-Ornithine; Metabolic engineering

摘要：L-Ornithine is a non-protein amino acid with extensive applications in medicine and the food industry. Currently, L-ornithine is produced by microbial fermentation; however, this process needs to be further improved in terms of L-ornithine productivity and cost reduction. In this study, overexpression of LysE was observed to increase L-ornithine production in engineered Corynebacterium glutamicum S9114. To overcome the drawbacks of using a plasmid to express LysE, P-tac, a strong promoter, was inserted in the upstream region of lysE on the chromosome. This strain was further engineered by attenuating the expression of ncgl2228 and proB, and enhancing the expression of gdh and argCJBD. Combination of those targets resulted in L-ornithine production at a titer of 25 g/L, which was 63.4% higher than that produced by the original strain (15.3 g/L). These results demonstrated the positive effects of overexpressing LysE on L-ornithine production and provided novel targets for developing L-ornithine-producing C. glutamicum strains.