文献类型：期刊文献

中文题名：用于检测细胞内谷胱甘肽的比率型荧光探针

英文题名：Ratiometric Fluorescent Probe for the Detection of Glutathione in Living Cells

作者：杨润洁[1];唐尧[1];朱维平[1]

机构：[1]华东理工大学药学院,上海市化学生物学重点实验室,上海200237

年份：2016

卷号：37

期号：4

起止页码：643

中文期刊名：高等学校化学学报

外文期刊名：Chemical Journal of Chinese Universities

收录：CSTPCD;;EI(收录号：20161902356004);Scopus;WOS:【SCI-EXPANDED(收录号:WOS:000372874200007)】；北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：Supported by the National Natural Science Foundation of China(No. 21476077).

语种：中文

中文关键词：荧光探针;谷胱甘肽;细胞成像

外文关键词：Fluorescent probe; Glutathione; Intracellular imaging;

摘要：设计合成了1种用于检测生物巯基的比率型荧光探针(4),并考察了其对谷胱甘肽的识别作用.在4-羟乙基哌嗪乙磺酸(HEPES)缓冲液中,探针4可与谷胱甘肽快速反应,溶液颜色由淡黄色变为粉红色,从而实现"裸眼"检测,且在608 nm处的荧光信号增强.在1.6×10^(-5)~2×10^(-4)mol/L范围内,探针4能够定量检测谷胱甘肽,检出限为8.9×10^(-7)mol/L.此外,探针4还可用于MCF-7细胞中谷胱甘肽的成像.
A fluorescent probe(4)for the detection of biothiols was designed and synthesized and its properties for labeling glutathione was investigated.After reaction with glutathione in 2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid(HEPES)buffer,the color of solution changed from light yellow to pink,which could be detected by naked eyes.Meanwhile,the fluorescence was enhanced at 608 nm.Probe 4 was useful for measuring glutathione at concentrations ranging from 1.6×10^(-5)to 2×10^(-4)mol/L with a detection limit of 8.9×10^(-7)mol/L.Besides,probe 4 was sensitive to glutathione in MCF-7 cells,which emitted red fluorescence when it was incubated with MCF-7 cells.