文献类型：期刊文献

英文题名：Salt stress causes cell wall damage in yeast cells lacking mitochondrial DNA

作者：Gao, Qiuqiang[1];Liou, Liang-Chun[2];Ren, Qun[2];Bao, Xiaoming[3];Zhang, Zhaojie[2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Univ Wyoming, Dept Zool & Physiol, Laramie, WY 82071 USA;[3]Shandong Univ, State Key Lab Microbial Technol, Jinan, Shandong, Peoples R China

年份：2014

卷号：1

期号：3

起止页码：94

外文期刊名：MICROBIAL CELL

收录：WOS:【ESCI(收录号:WOS:000218667400003)】；

基金：This study was supported in part by an Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under grant number P30 GM103398, by the National Natural Science Foundation of China (No. 31300070), and the China Postdoctoral Science Foundation (No. 2012M520850).

语种：英文

外文关键词：cell wall damage; reactive oxygen species; SCW11; salt stress; Saccharomyces cerevisiae

摘要：The yeast cell wall plays an important role in maintaining cell morphology, cell integrity and response to environmental stresses. Here, we report that salt stress causes cell wall damage in yeast cells lacking mitochondrial DNA (rho(0)). Upon salt treatment, the cell wall is thickened, broken and becomes more sensitive to the cell wall- perturbing agent sodium dodecyl sulfate (SDS). Also, SCW11 mRNA levels are elevated in rho(0) cells. Deletion of SCW11 significantly decreases the sensitivity of rho(0) cells to SDS after salt treatment, while overexpression of SCW11 results in higher sensitivity. In addition, salt stress in rho(0) cells induces high levels of reactive oxygen species (ROS), which further damages the cell wall, causing cells to become more sensitive towards the cell wall- perturbing agent.