文献类型：期刊文献

英文题名：Enhanced Healing of Rat Calvarial Defects with Sulfated Chitosan-Coated Calcium-Deficient Hydroxyapatite/Bone Morphogenetic Protein 2 Scaffolds

作者：Zhao, Jun[1];Shen, Gang[2];Liu, Changsheng[3];Wang, Shaoyi[1];Zhang, Wenjie[4];Zhang, Xiaochen[1];Zhang, Xiuli[5];Ye, Dongxia[5];Wei, Jie[3];Zhang, Zhiyuan[1];Jiang, Xinquan[4,5]

机构：[1]Shanghai Jiao Tong Univ, Sch Dent Med, Peoples Hosp 9, Dept Oral & Maxillofacial Surg,Coll Stomatol, Shanghai 200011, Peoples R China;[2]Shanghai Jiao Tong Univ, Sch Dent Med, Peoples Hosp 9, Dept Orthodont,Coll Stomatol, Shanghai 200011, Peoples R China;[3]E China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, Engn Res Ctr Biomed Mat, Shanghai 200237, Peoples R China;[4]Shanghai Jiao Tong Univ, Sch Med, Peoples Hosp 9, Dept Prosthodont,Coll Stomatol, Shanghai 200011, Peoples R China;[5]Shanghai Jiao Tong Univ, Sch Med, Peoples Hosp 9, Shanghai Res Inst Stomatol,Shanghai Key Lab Stoma, Shanghai 200011, Peoples R China

年份：2012

卷号：18

期号：1-2

起止页码：185

外文期刊名：TISSUE ENGINEERING PART A

收录：;EI(收录号：20120214668867);WOS:【SCI-EXPANDED(收录号:WOS:000299111500018)】；

基金：This work was supported by National Natural Science Foundation of China 30973342, 30973363. Program for New Century Excellent Talents in University NCET-08-0353. Science and Technology Commission of Shanghai Municipality S30206, 0952nm04000, 10430710900, 10dz2211600, 10ZR1418100. Shanghai Education Committee 07SG19.

语种：英文

外文关键词：Biocompatibility - Bone - Bone cement - Calcium phosphate - Chitosan - Coatings - Computerized tomography - Defects - Degradation - Hydroxyapatite - Proteins - Scaffolds (biology)

摘要：Calcium phosphate cements (CPCs), which are widely used in bone regeneration, possess good biocompatibility and osteoconductivity and have been demonstrated to be candidate carriers for bone growth factors. However, limited release of growth factors from CPCs and slow degradation of the materials are not desirable for certain clinical applications. Previous studies have shown that calcium-deficient hydroxyapatite (CDHA) from CPCs presents more rapid degradation rate than CPCs. In this study, a hybrid growth factor delivery system was prepared by using bone morphogenetic protein 2 (BMP-2) loaded CDHA porous scaffold with sulfated chitosan (SCS) coating for improved release profile. We tested the BMP-2 release characteristic of CDHA/BMP-2/SCS composite in vitro and its ability to repair rat calvarial bone defects. A higher percentage of BMP-2 was released when sulfated chitosan coating was present compared with CDHA/BMP-2 group. Eight weeks postoperation, the repaired crania were evaluated by microcomputed tomography, sequential fluorescent labeling, histological analysis, and immunohistochemistry. CDHA/BMP-2/SCS group promoted the most extensive new bone formation than CDHA/BMP-2 and CDHA groups. Our observations suggest that sulfated chitosan coating could enhance the release profile of CDHA/BMP-2 composite in vitro and promote new bone formation in vivo. The hybrid CDHA/BMP-2/SCS system is a promising growth factor delivery strategy for bone regeneration.