文献类型：期刊文献

英文题名：Cellulosic L-Lactic Acid Production by Engineered Na+-Tolerant Pediococcus acidilactici and Bipolar Membrane Electrodialysis Purification for L-Lactide Synthesis

作者：Li, Zhibin[1]; Qu, Chaolong[1]; Zhu, Tao[1]; Han, Qingmei[1]; Zhang, Bin[1]; Bao, Jie[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China

年份：2026

外文期刊名：Biotechnology and Bioengineering

收录：EI(收录号：20261220328765)

语种：英文

外文关键词：Cellulose - Electric power utilization - Feedstocks - Membranes - Purification - Recovery - Sodium hydroxide - Solid wastes - Sulfur compounds

摘要：Ultra-pure L-lactic acid is a crucial precursor for the synthesis of L-lactide and polylactic acid (PLA). Regular purification of L-lactic acid results in a low L-lactic acid recovery yield and CaSO4 solid waste generation. This study used highly simplified bipolar membrane electrodialysis for purification of sodium L-lactic acid using wheat straw feedstock, taking advantage of sharply reduced electricity cost from rapid development of photovoltaic and wind power. An engineered L-lactic acid bacterium, Pediococcus acidilactici HL2 with high Na+ tolerance was constructed by co-expression of heterologous Na+/H+ antiporter and native β-component of ATPase to meet the needs of bipolar membrane electrodialysis for soluble sodium L-lactate. The bipolar membrane electrodialysis for the cellulosic sodium L-lactate purification broths received 94.6% of Na+ removal, and the overall recovery yield was 80.2%, considerably higher than regular purification methods. 80% of sodium hydroxide was recovered after electrodialysis and recycled for pH adjustment in fermentation. The electric power consumption was calculated as 1.43 kWh per kg of pure L-lactic acid, which is merely $50 per metric ton of L-lactic acid based on the current price of green power. The L-lactide synthesized using the purified L-lactic acid successfully met the standard of commercial lactide for PLA polymerization. This study provides a green and sustainable procedure for L-lactide synthesis from lignocellulose feedstocks by eliminating sulfuric acid addition, avoiding solid waste generation, and recycling neutralizer for fermentation. ? 2026 Wiley Periodicals LLC.