文献类型：期刊文献

英文题名：The Online Morphology Control and Dynamic Studies on Improving Vitamin B12 Production by Pseudomonas denitrificans with Online Capacitance and Specific Oxygen Consumption Rate

作者：Wang, Ze-Jian[1,2,3];Shi, Hui-lin[1,2];Wang, Ping[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai Inst Biomfg Technol, POB 329,130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Collaborat Innovat Ctr, POB 329,130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Delft Univ Technol, Dept Biotechnol, Julianalaan 67, NL-2628 BC Delft, Netherlands

年份：2016

卷号：179

期号：6

起止页码：1115

外文期刊名：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

收录：;EI(收录号：20161402187080);WOS:【SCI-EXPANDED(收录号:WOS:000381157400015)】；

基金：This work was financially supported by a grant from the National Natural Science Foundation of China (Grant No. 31200024), the Royal DSM, and partially supported by the NOW-MoST Joint Program (2013DFG32630).

语种：英文

外文关键词：Morphology; Capacitance; Specific oxygen consumption rate; Vitamin B-12; Pseudomonas denitrificans

摘要：The relationship between the morphological character of Pseudomonas denitrificans and vitamin B-12 synthesis based on real-time capacitance measurement and online specific oxygen consumption rate (Q (O2)) control was established for enhancing vitamin B-12 production. Results demonstrated that the threshold Q (O2) value lower than 2.0 mmol/gDCW/l would greatly stimulate the state transfer from the cell number growth phase to the cell elongation phase and promote rapid vitamin B-12 biosynthesis, while the vitamin B-12 biosynthesis rate could also be inhibited when the rate of cell's length-to-width ratio (ratio-LW) was higher than 10:1. Furthermore, the optimal morphology controlling strategy was achieved based on online Q (O2) control, which increases the appropriate active cell numbers at the former phase, and then control the elongation of ratio-LW no more than 10:1 at the vitamin B-12 biosynthesis phase. The maximal vitamin B-12 production reached 239.7 mg/l at 168 h, which was improved by 14.7 % compared with the control (208 mg/l). This online controlling strategy would be effectively applied for improving industrial vitamin B-12 fermentation.