文献类型：期刊文献

英文题名：Peptide T7-modified polypeptide with disulfide bonds for targeted delivery of plasmid DNA for gene therapy of prostate cancer

作者：Lu, Yue[1];Jiang, Wenjun[1];Wu, Xin[2];Huang, Saixu[1];Huang, Zhiyong[1];Shi, Yamin[3];Dai, Qi[1];Chen, Jianming[2];Ren, Fuzheng[1];Gao, Shen[4]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab New Drug Design, Sch Pharm, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Weier Biol Med Sci & Technol Co Ltd, Shanghai, Peoples R China;[3]Fujian Univ Tradit Chinese Med, Dept Pharm, Fuzhou, Fujian, Peoples R China;[4]Second Mil Med Univ, Dept Pharmaceut, Changhai Hosp, Shanghai, Peoples R China

年份：2018

卷号：13

起止页码：6913

外文期刊名：INTERNATIONAL JOURNAL OF NANOMEDICINE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000449040700001)】；

基金：This study was sponsored by the National Natural Science Foundation of China (grant no 81772749) and Shanghai Rising-Star Program (grant no 18QB1400400).

语种：英文

外文关键词：arginine peptide; aspartic acid peptide; tumor targeting; DNA delivery; bone metastasis prostate cancer

摘要：Badcground: Vectors are essential for successful gene delivery. In the present study, a tumor-targeting cationic gene vector, known as the disulfide cross-linked arginine-aspartic acid peptide modified by HAIYPRH (T7) peptide (CRD-PEG-T7), was designed for targeted delivery of plasmid DNA (pDNA) for gene therapy of prostate cancer (PCa). Methods: The structure of CRD-PEG-T7 was determined and the cellular uptake efficacy, gene transfection efficacy, cytotoxicity, and the targeting effect of the CRD-PEG-T7-plasmid DNA complex were examined. Results: The results demonstrated that the CRD-PEG-T7-plasmid DNA complex was nanosized and had a positively charged surface, good cellular uptake efficacy, minimal cytotoxicity, and a dual-targeting effect as compared with the CRD-PEG-plasmid DNA complex. The peptide T7-modifed new delivery system was able to target the highly expressed transferrin receptor (Till) on tumor cells with an efficiency four-fold higher than that of the non-modified system. Conclusion: The results above indicatd that the CRD-PEG-T7-plasmid DNA complex may prove to be a promising gene delivery system targeting bone-metastatic tumor.