文献类型：期刊文献

英文题名：Biodegradation of phthalate esters by newly isolated Rhizobium sp LMB-1 and its biochemical pathway of di-n-butyl phthalate

作者：Tang, W. -J.[1];Zhang, L. -S.[1];Fang, Y.[1];Zhou, Y.[1];Ye, B. -C.[1]

机构：[1]East China Univ Sci & Technol, Lab Biosyst & Microanal, State Key Lab Bioreactor Engn, Meilong RD 130, Shanghai 200237, Peoples R China

年份：2016

卷号：121

期号：1

起止页码：177

外文期刊名：JOURNAL OF APPLIED MICROBIOLOGY

收录：;EI(收录号：20231713968967);WOS:【SCI-EXPANDED(收录号:WOS:000379535400015)】；

基金：This research was supported by National Science Foundation of China (31401592).

语种：英文

外文关键词：biodegradation; GC-MS; pathway; phthalates; Rhizobium sp; LMB-1

摘要：AimsTo isolate a novel strain that could degrade many kinds PAEs efficiently and investigate the DBP-degrading pathway in this strain. Methods and ResultsBased on its 16S rRNA gene sequence, the strain was identified as Rhizobium sp. This strain, named LMB-1, can also utilize phthalates, such as DEHP, DMP, DBP and DEP. During the degradation of DBP, six possible metabolites, diethyl phthalate, mono-ethyl phthalate, di-methyl phthalate, mono-methyl phthalate, phthalic acid and tartaric acid, were identified by gas chromatography-mass spectrometry (GC-MS) analysis, and the degradation pathway of DBP was also identified in this study. ConclusionsIn summary, strain LMB-1, identified as Rhizobium sp., was found to be capable of efficiently degrading PAEs, and it was determined that the strain degraded DMP completely within 45h. DEP, DMP, MEP, MMP, PA and tartaric acid were detected during the course of DBP degradation by LMB-1. We propose that this strain could completely degrade DBP or other PAEs. Our results offer a novel and potential candidate, Rhizobium sp. LMB-1, for use in the bioremediation of cultivated soil contaminated by PAEs. Significance and Impact of the StudyThis is the first report concerning the complete degradation of phthalate esters by Rhizobium sp.