文献类型：期刊文献

英文题名：A highly luminescent lanthanide-functionalized covalent organic framework for rapid and specific detection of 7-methylguanine for DNA methylation assessment

作者：Hu, Tao[1];Hao, Ji-Na[1];Li, Yongsheng[1,2]

机构：[1]East China Univ Sci & Technol, Sch Mat Sci & Engn, Lab Low Dimens Mat Chem, Key Lab Ultrafine Mat,Minist Educ,Frontier Sci Ctr, Shanghai 200237, Peoples R China;[2]Shihezi Univ, Sch Chem & Chem Engn, Key Lab Green Proc Chem Engn Xinjiang Bingtuan, Shihezi 832003, Peoples R China

年份：2024

卷号：12

期号：20

起止页码：5266

外文期刊名：BIOMATERIALS SCIENCE

收录：;EI(收录号：20243717031757);WOS:【SCI-EXPANDED(收录号:WOS:001307838200001)】；

基金：This work was financially supported by the National Key Research and Development Program of China (Grant No. 2022YFC2403203), the National Natural Science Foundation of China (No. 52172279), the Basic Research Program of Shanghai (21JC1406003), the Shanghai Rising-Star Program (21QA1402200), the Natural Science Foundation of Shanghai (21ZR1416600), the Leading Talents in Shanghai in 2018, and the Higher Education Discipline Innovation Project (B14018).

语种：英文

外文关键词：Alkylation - Biomarkers - Body fluids - Methylation

摘要：Rapid and accurate detection of 7-methylguanine (m(7)Gua), a biomarker reflecting the degree of DNA methylation that occurs before or in the early stage of cancer, is of particular significance but remains a great challenge. Herein, a luminescent lanthanide-based covalent organic framework (Ln-COF) probe, namely DPA/Eu@ETTA-DHTA, is designed for the first time for the identification of m(7)Gua by assembling pyridine-2,6-dicarboxylic acid (DPA) as both an energy donor and a recognition molecule and Eu3+ ions as signal reporters into a stable COF matrix with high porosity and available binding sites. Significantly, the characteristic luminescence of Eu3+ ions can be turned on by the grafted DPA in the COF probe and effectively quenched by the addition of m(7)Gua via a competitive absorption process, thus achieving the sensing of m(7)Gua. Such a Ln-COF-based fluorescent platform presents high selectivity and a rapid response (<1 min) to m(7)Gua with a low detection limit (mu M level) even in the presence of the main coexisting species in urine, allowing it to serve as a potentially practical probe for point-of-care monitoring of the level of m(7)Gua in human urine specimens. This study provides a convenient, time-saving, and economical approach for visual detection of m(7)Gua, and opens up new perspectives for the design of a luminescent COF-based probe for DNA methylation evaluation in diagnostics.