文献类型：专利

英文题名：Gene encoding nicotinamide adenine dinucleotide phosphoric acid fluorescent probe in kit used for detecting or screening drug, comprises nicotinamide adenine dinucleotide phosphate-sensitive polypeptide B and fluorescent protein A

作者：LIU H;YANG Y;CHU H;FAN C;YAO R

机构：[1]UNIV EAST CHINA SCI & TECHNOLOGY

申请号：CN104910276-A

申请日：2014-03-14

公开日：2015-09-16

语种：英文

收录：DERWENT

摘要：NOVELTY - Gene encoding nicotinamide adenine dinucleotide phosphoric acid fluorescent probe comprises nicotinamide adenine dinucleotide phosphate (NADH)-sensitive polypeptide B and fluorescent protein A. Interaction between the polypeptide B and fluorescent protein A leads to change in fluorescence intensity. USE - Gene encoding nicotinamide adenine dinucleotide phosphoric acid fluorescent probe in kit used for detecting or screening drug (all claimed). DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for: (1) a method for preparing fluorescent probe, which involves inserting the nucleotide sequence into expression vector; transferring the expression vector into host cell; culturing the host cell; and separating the fluorescent probe from the host cell; and (2) a kit, which comprises Gene encoding nicotinamide adenine dinucleotide phosphoric acid fluorescent probe.