文献类型：期刊文献

英文题名：Simultaneous determination of tocopherols and tocotrienols in vegetable oils by GC-MS

作者：Zhang, Rui[1];Shen, Weijian[1];Wei, Xueyuan[2];Zhang, Feifang[3];Shen, Chongyu[1];Wu, Bin[1];Zhao, Zengyun[1];Liu, Han[1];Deng, Xiaojun[4]

机构：[1]Jiangsu Entry Exit Inspect & Quarantine Bur, Food Lab, Nanjing 210001, Jiangsu, Peoples R China;[2]China Pharmaceut Univ, Coll Pharm, Nanjing 210009, Jiangsu, Peoples R China;[3]East China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China;[4]Shanghai Entry Exit Inspect & Quarantine Bur, Tech Ctr Anim Plant & Food Inspect & Quarantine, Shanghai 200135, Peoples R China

年份：2016

卷号：8

期号：40

起止页码：7341

外文期刊名：ANALYTICAL METHODS

收录：;EI(收录号：20164302930027);WOS:【SCI-EXPANDED(收录号:WOS:000386221000007)】；

基金：This research is financially supported by the Science and Technology Project of AQSIQ (No. 2015IK141) and the Science and Technology Project of Shanghai Yangtze River Delta (No. 15395810100).

语种：英文

外文关键词：Olive oil - Mass spectrometry - Ultrasonic applications

摘要：A simple and rapid gas chromatography-mass spectrometry (GC-MS) method was developed and validated for the simultaneous determination of alpha-, beta-, gamma- and delta-tocopherols and alpha-, beta-, gamma- and delta-tocotrienols in vegetable oils. In this study, tedious sample pretreatments like saponification and derivatization, which are commonly used in previously published related methods, were not adopted. Tocopherols and tocotrienols were isolated from oil samples by ultrasonic extraction with methanol, then centrifuged and the supernatant was directly injected into the GC-MS system for analysis. The results showed that alpha-, beta-, gamma- and delta-tocopherols and alpha-, beta-, gamma- and delta- tocotrienols were well separated within 14 minutes. The instrumental limits of detection and quantification ranged from 0.3 to 2.5 ng mL(-1) and from 1.0 to 8.3 ng mL(-1), respectively. Calibration curves were well correlated (r(2) > 0.99) within the concentration range of 10 to 1000 ng mL(-1) for all eight compounds. Recovery tests were carried out by spiking virgin olive oil with standards at three concentration levels (10, 50 and 250 mg kg(-1)), in which the average recoveries (n = 6) varied from 83.7% to 117.2%, with relative standard deviations ranging between 1.9% and 7.5%. The proposed method proved to be simple, sensitive and accurate, and was successfully applied to the determination of tocopherols and tocotrienols in vegetable oils.