文献类型：期刊文献

英文题名：Laminin-coated nerve guidance conduits based on poly(L-lactide-co-glycolide) fibers and yarns for promoting Schwann cells' proliferation and migration

作者：Wu, Tong[1];Li, Dandan[2];Wang, Yuanfei[3];Sun, Binbin[1];Li, Dawei[4];Morsi, Yosry[5];El-Hamshary, Hany[6,7];Al-Deyab, Salem S.[6];Mo, Xiumei[1,2]

机构：[1]Donghua Univ, Coll Chem Chem Engn & Biotechnol, State Key Lab Modificat Chem Fibers & Polymer Mat, 2999 North Renmin Rd, Shanghai 201620, Peoples R China;[2]Donghua Univ, Coll Mat Sci & Engn, Shanghai 201620, Peoples R China;[3]East China Univ Sci & Technol, Sch Resources & Environm Engn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[4]Donghua Univ, Coll Text, Shanghai 201620, Peoples R China;[5]Swinburne Univ Technol, Fac Engn & Ind Sci, Hawthorn, Vic 3122, Australia;[6]King Saud Univ, Dept Chem, Coll Sci, Riyadh 11451, Saudi Arabia;[7]Tanta Univ, Dept Chem, Fac Sci, Tanta 31527, Egypt

年份：2017

卷号：5

期号：17

起止页码：3186

外文期刊名：JOURNAL OF MATERIALS CHEMISTRY B

收录：;EI(收录号：20172803923402);WOS:【SCI-EXPANDED(收录号:WOS:000400501900012)】；

基金：This research was supported by the National Major Research Program of China (2016YFC1100202), National Natural Science Foundation of China (31470941, 31271035), Science and Technology Commission of Shanghai Municipality (15JC1490100, 15441905100), Fundamental Research Funds for the Central Universities (CUSF-DH-D-2017047), PhD Programs Foundation of Ministry of Education of China (20130075110005) and light of textile project (J201404). The authors would like to extend their sincere appreciation to the Deanship of Scientific Research at King Saud University for its funding through the research group project no. RGP-201.

语种：英文

外文关键词：Topology - Tissue engineering - Electrospinning - Fibers - Neurons - Coatings - Wool

摘要：To develop an effective nerve guidance conduit with cooperative effects of topological structure and biological cues for promoting Schwann cells' (SCs) proliferation and migration, a laminin-coated and yarn-encapsulated poly(L-lactide-co-glycolide) (PLGA) nerve guidance conduit (LC-YE-PLGA NGC) was fabricated in this study. The PLGA fiber yarns were fabricated through a double-nozzle electrospinning system and then the PLGA fibrous outer layer was collected using a general electrospinning method. Subsequently, laminin was coated on the yarn-encapsulated PLGA NGC through covalent binding. The results showed satisfactory tensile mechanical strength of the laminin-coated PLGA fibers/yarns and good compressive mechanical support of the LC-YE-PLGA NGC. SCs proliferation was significantly superior (p < 0.05) on the PLGA and laminin-coated PLGA yarns than the PLGA fibers. Furthermore, the LC-YE-PLGA NGC performed much better in SCs migration compared with the NGCs without yarn-encapsulation or laminin-coating, indicating the synergistic effect of the three-dimensional yarn structure (topological structure) and the laminin-coating (biological cues) for SCs proliferation and migration. Therefore, the LC-YE-PLGA NGC demonstrated promising potential in promoting SCs proliferation and inducing SCs migration in nerve tissue engineering.