文献类型：期刊文献

英文题名：Investigation of Lipid Metabolism in Dynamic Progression of Coronary Artery Atherosclerosis of Humans by Time-of-Flight Secondary Ion Mass Spectrometry

作者：Li, Hao-Wen[1,2];Hu, Zhan[3,4];Chen, Xiao[3,4];Ren, Jie[3,4];Cui, Hao[3,4];Zhang, Min[1];Chen, Liang[3,4];Hua, Xin[1,5];Song, Jiangping[3,4];Long, Yi-Tao[6]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, Renji Hosp, Sch Med,Shanghai Key Lab Nucle Acid Chem & Nanome, Inst Mol Med,State Key Lab Oncogenes & Related Ge, Shanghai 200127, Peoples R China;[3]Fuwai Hosp, State Key Lab Cardiovasc Dis, Beijing 100037, Peoples R China;[4]Chinese Acad Med Sci CAMS & Perking Union Med Col, Natl Ctr Cardiovasc Dis, Beijing 100037, Peoples R China;[5]Southeast Univ, Sch Chem & Chem Engn, Jiangsu Engn Lab Smart Carbon Rich Mat & Device, Nanjing 211189, Peoples R China;[6]Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, Nanjing 210023, Peoples R China

年份：2021

卷号：93

期号：8

起止页码：3839

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20210909984189);WOS:【SCI-EXPANDED(收录号:WOS:000626269400024)】；

基金：This research was supported by Beijing Nova Program (Z201100006820003), the Fundamental Research Funds for the Central Universities (3332018048) and National Natural Science Foundation of China (81800398, 21705046).

语种：英文

外文关键词：Diseases - Spatial distribution - Magnetic resonance - Metabolites - Tissue - Stochastic systems - Biomolecules - Linoleic acid - Lipids - Metabolism - Secondary ion mass spectrometry - Secondary emission

摘要：Alterations in lipid metabolites in coronary artery tissues are phenotypic changes in the progression of atherosclerosis (AS). A full picture of the spatiotemporal distribution of lipid metabolites in coronary AS is needed for a deeper understanding of its pathology and the identification of potential biomarkers of disease progression. In this work, the changes in species, quantity, and distribution of lipid metabolites at different stages of AS, which were standardized by the disease areas, were analyzed through the high spatial resolution- and high sensitivity-time-of-flight secondary ion mass spectrometry (ToF-SIMS) under delayed extraction mode. Based on high lateral resolution imaging, we further analyzed the ToF-SIMS data extracted from the subregions of AS lesion tissues at different disease progression stages by semiquantitative comparison, clustering analysis (t-stochastic neighbor embedding and HCA), and KEGG enrichment. Thus, a much-detailed description of lipids' features in coronary AS was achieved. We constructed a ToF-SIMS mass spectrometry database of coronary AS lipids. 40 specific lipid metabolites with distinctive patterns between different pathological stages were obtained. Chemical imaging unveiled further details regarding the spatial distribution of lipids. Moreover, linoleic acid and arachidonic acid metabolic pathway were predicted to be critical in AS progression.