文献类型：期刊文献

英文题名：Evaluation and application of a mixed-mode chromatographic stationary phase in two-dimensional liquid chromatography for the separation of traditional Chinese medicine

作者：Wei, Zhishen[1];Fu, Qing[1];Cai, Jianfeng[1];Huan, Liyun[1];Zhao, Jianchao[1];Shi, Hui[1];Jin, Yu[1];Liang, Xinmiao[1]

机构：[1]East China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Sch Pharm, Minist Educ, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2016

卷号：39

期号：12

起止页码：2221

外文期刊名：JOURNAL OF SEPARATION SCIENCE

收录：;EI(收录号：20162602547235);WOS:【SCI-EXPANDED(收录号:WOS:000379262700002)】；

基金：This work was supported by the Natural Science Foundation of Shanghai, China (Grant no. 13ZR1453200).

语种：英文

外文关键词：Mixed-mode chromatography; Stationary phases; Traditional Chinese medicine; Two-dimensional liquid chromatography

摘要：In this study, two mixed-mode chromatography stationary phases (C8SAX and C8SCX) were evaluated and used to establish a two-dimensional liquid chromatography system for the separation of traditional Chinese medicine. The chromatographic properties of the mixed-mode columns were systematically evaluated by comparing with other three columns of C8, strong anion exchanger, and strong cation exchanger. The result showed that C8SAX and C8SCX had a mixed-mode retention mechanism including electrostatic interaction and hydrophobic interaction. Especially, they were suitable for separating acidic and/or basic compounds and their separation selectivities could be easily adjusted by changing pH value. Then, several off-line 2D-LC systems based on the C8SAX in the first dimension and C8SAX, C8SCX, or C8 columns in the second dimension were developed to analyze a traditional Chinese medicine-Uncaria rhynchophylla. The two-dimensional liquid chromatography system of C8SAX (pH 3.0) x C8SAX (pH 6.0) exhibited the most effective peak distribution. Finally, fractions of U. rhynchophylla prepared from the first dimension were successfully separated on the C8SAX column with a gradient pH. Thus, the mixed-mode stationary phase could provide a platform to separate the traditional Chinese medicine in practical applications.