文献类型：期刊文献

英文题名：Multistimulative Nanogels with Enhanced Thermosensitivity for Intracellular Therapeutic Delivery

作者：Ji, Ping[1];Zhou, Bingjie[2,3];Zhan, Yuan[1];Wang, Yifeng[2,3];Zhang, Yuhong[1];Li, Yulin[1,2,3,4];He, Peixin[1]

机构：[1]Hubei Univ, Key Lab Synth & Applicat Organ Funct Mol, Hubei Collaborat Innovat Ctr Adv Organ Chem Mat, Minist Educ,Coll Chem & Chem Engn, Wuhan 430062, Hubei, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, Engn Res Ctr Biomed Mat, Shanghai 200237, Peoples R China;[4]Univ Madeira, MMRG, CQM, Campus Univ Penteada, P-9020105 Funchal, Portugal

年份：2017

卷号：9

期号：45

起止页码：39143

外文期刊名：ACS APPLIED MATERIALS & INTERFACES

收录：;EI(收录号：20174704443830);WOS:【SCI-EXPANDED(收录号:WOS:000416203800006)】；

基金：The present study was financially supported by grants from the Shanghai Municipal Natural Science Foundation (15ZR1408500), the Shanghai International Cooperation Program (15520721200), and the Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Ministry of Education (2014-KL-004).

语种：英文

外文关键词：stimulative nanogels; alginate; poly(N-isopropylacrylamide); temperature-induced cell death; drug delivery

摘要：The flexibility and hydrophilicity of nanogels suggest their potential for the creation of nanocarriers with good colloidal stability and stimulative ability. In the present study, biocompatible AGP and AGPA nanogels with triple-stimulative properties (thermo-sensitivity, pH sensitivity, and redox sensitivity) were prepared by incorporating poly(N-isopropylacrylamide) (PNIPAM) or poly(N-isopropylacrylamide-co-acrylic acid) (P(NIPAM-AA)) into alginate (AG) emulsion nanodrops, followed by fixation with a disulfide containing molecule (cystamine dihydrochloride (Cys)). Compared to AG/PNIPAM(AGP) nanogels, AG/P(NIPAM-AA) (AGPA) nanogels exhibited more sensitive volumetric expansion by switching the temperature from 40 to 25 degrees C under physiological medium. This expansion occurs because P(NIPAM-AA) with -COOH groups can be fixed inside the nanogels via chemical bonding with Cys, whereas PNIPAM was encapsulated in the nanogels through simple physical interactions with the AG matrix. AGPA nanogels carrying an anticancer drug tend to easily enter cells upon heating, thereby exerting toxicity through a cold shock and reverse thermally induced release of an anticancer drug. Upon internalization inside cells, the nanogels use the reducible and acidic intracellular environments to effectively release the drug to the nucleus to impart anticancer activity. These results demonstrate that multifunctional nanogels may be used as a general platform for therapeutic delivery.