文献类型：期刊文献

英文题名：A novel high-copy plasmid, pEC, compatible with commonly used Escherichia coli cloning and expression vectors

作者：Zhang, Huijing[1]; Wu, Haizhen[1]; Zhang, Huizhan[1]

机构：[1] State Key Laboratory of Bioreactor Engineering [Mailbox 431], East China University of Science and Technology, Shanghai 200237, China

年份：2007

卷号：29

期号：3

起止页码：431

外文期刊名：Biotechnology Letters

收录：EI(收录号：20070910462149)

语种：英文

外文关键词：Biomarkers - Cells - Cloning - Genes

摘要：A 66164-bp cryptic plasmid, pEIB1, was isolated from strain Vibrio anguillarum MVM425 and sequenced. A plasmid carrying a 1089-bp fragment, containing the minimal replication region of pEIB1, a kanamycin-resistance marker and an l-arabinose promoter, designated pEC, was maintained as a high copy plasmid in E. coli and stably inherited in the absence of antibiotic selection. Significantly, pEC was compatible with the widely used ColE1, pSC101 and p15A replicons making it a useful tool for a dual-plasmid expression system. ? 2006 Springer Science+Business Media B.V.