文献类型：期刊文献

英文题名：Sensing Cellular Damages Induced by Food Safety Hazards Using Bacterial Stress-Responsive Biosensors

作者：Li, Ruiqi[1,2];Lou, Manzhuan[2,3];He, Wei[4];Quan, Shu[1,2,3]

机构：[1]East China Univ Sci & Technol, Sch Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, Zhangjiang Inst Adv Study, Shanghai 201203, Peoples R China;[3]Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China;[4]Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Ctr Excellence Mol Cell Sci, State Key Lab Mol Biol, Shanghai 200031, Peoples R China

年份：2025

卷号：15

期号：10

外文期刊名：BIOSENSORS-BASEL

收录：;EI(收录号：20254419421700);WOS:【SCI-EXPANDED(收录号:WOS:001601840900001)】；

基金：This work was supported by the National Key Research and Development Program of China, Grant Number: 2022YFF1102900; The National Natural Science Foundation of China (NSFC), Grant Numbers: 32222049, 32201043, and 32171269; The Natural Science Foundation of Shanghai, Grant Number: 23ZR1415300; The Shanghai Frontier Science Center of Optogenetic Techniques for Cell Metabolism, Shanghai Municipal Education Commission, Grant Number: 2021 Sci & Tech 03 28; Shanghai Municipal Science and Technology Major Project.

语种：英文

外文关键词：whole-cell biosensor; cellular damage detection; food safety hazards; stress-responsive biosensors

摘要：Food safety hazards induce diverse cellular damages including DNA damage, oxidative stress, proteotoxic stress, and membrane disruption, ultimately contributing to various human diseases. Conventional toxicity assays, while effective, are often resource-intensive and lack the capacity to distinguish among these different damage types, thereby limiting insight into toxic responses and the development of effective strategies for targeted risk mitigation. Here, we constructed a panel of Escherichia coli whole-cell biosensors capable of distinguishing distinct categories of cellular damage. Specifically, an optimized RecA-LexA-based DNA damage biosensor that precisely controls the exogenous expression of the transcriptional repressor LexA achieved a 35.5% reduction in baseline signal and a 36.6-fold induction of fluorescence. In parallel, systematic promoter screening identified Pfpr, PkatG, PgrpE, and PfabA as effective modules for constructing oxidative, proteotoxic, and membrane stress biosensors. These biosensors exhibited high specificity and sensitivity, generating dose-dependent responses to model toxicants and enabling discrimination of cellular damage induced by typical hazards such as norfloxacin and ciprofloxacin. Notably, the DNA damage biosensor detected norfloxacin with a limit of detection (LOD) of 1.3 ng/mL in standard solution and 3.0 ng/mL in milk, comparable to that of high-performance liquid chromatography (HPLC). Together, our work not only provides a versatile, cost-effective, and sensitive tool for assessing diverse cellular damages induced by food safety hazards, but also demonstrates potential utility for practical food safety monitoring.