文献类型：期刊文献

英文题名：Preparation and chromatographic evaluation of a newly designed steviol glycoside modified-silica stationary phase in hydrophilic interaction liquid chromatography and reversed phase liquid chromatography

作者：Liang, Tu[1];Fu, Qing[1];Shen, Aijin[2];Wang, Hui[1];Jin, Yu[1];Xin, Huaxia[1];Ke, Yanxiong[1];Guo, Zhimou[2];Liang, Xinmiao[1,2]

机构：[1]E China Univ Sci & Technol, Sch Pharm, Engn Res Ctr Pharmaceut Proc Chem, Minist Educ, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Nat Med, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

年份：2015

卷号：1388

起止页码：110

外文期刊名：JOURNAL OF CHROMATOGRAPHY A

收录：;EI(收录号：20150900578165);WOS:【SCI-EXPANDED(收录号:WOS:000351792400013)】；

基金：This work was supported by China Postdoctoral Science Foundation (grant no. 2013M541481) and the Natural Science Foundation of Shanghai, China (grant no. 13ZR1453200).

语种：英文

外文关键词：Rebaudioside A; Stationary phase; Hydrophilic interaction liquid chromatography (HILIC); Mixed-mode; Thiol-ene click chemistry

摘要：A diterpene glycoside compound, rebaudioside A (commonly abbreviated as RA), was immobilized onto porous silica surface through "thiol-ene" click chemistry strategy. The successful immobilization of the RA on the silica support was confirmed by FT-IR and elemental analysis. Chromatographic characteristics of the new stationary phase, named Click TE-RA, were evaluated by a set of diverse analytes such as carbohydrates, nucleosides, and organic acids in hydrophilic interaction liquid chromatography (HILIC) mode. The effects of water content, buffer pH and concentration were investigated and a typical HILIC retention feature of Click TE-RA was observed at high organic modifier content. The Click TE-RA stationary phase was further studied by a series of glycoside compounds. Tunable retention mechanisms from hydrophilic to hydrophobic interactions were observed. Separation of very polar compounds including oligosaccharides, nucleic acid bases and nucleosides using Click TE-RA in HILIC mode was successfully accomplished. In addition, separation of saponins both in HILIC and reversed-phase liquid chromatography (RPLC) modes was performed, demonstrating the presence of orthogonality between two different modes on Click TE-RA column. The multiple interactions induced by polar sugar group and hydrophobic aglycone group allowed this Click TE-RA to serve as a multi-mode stationary phase in two-dimensional liquid chromatography. (C) 2015 Elsevier B.V. All rights reserved.