文献类型：期刊文献

中文题名：云芝糖肽的硼酸亲和色谱纯化

英文题名：Purification of Polysaccharide Peptide of Coriolus versicolor by Borate Affinity Chromatography

作者：查娟[1];刘坐镇[1];邬行彦[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2003

卷号：34

期号：2

起止页码：66

中文期刊名：中国医药工业杂志

外文期刊名：Chinese Journal of Pharmaceuticals

收录：CSTPCD;;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金项目 ( 2 970 6 0 0 2 )

语种：中文

中文关键词：云芝糖肽;硼酸;亲和色谱;纯化;彩绒革盖菌

外文关键词：affinity chromatography; polysaccharide peptide; purification

摘要：应用硼酸亲和色谱纯化云芝糖肽 ,最佳吸附条件为含 0 .4m ol/ L Na Cl的 0 .2 m ol/ L 磷酸盐缓冲液 (p H8.5 ) ,最佳洗脱条件为 0 .2 mol/ L 的硼酸溶液。纯化后 ,糖含量提高 6 6 % ,蛋白含量提高 2 .6 5倍 ,蛋白 -糖之比从 0 .31提高到 0 .6
The purification of polysaccharide peptide of Coriolus versicolor by borate affinity chromatography was introduced. The optimal adsorption condition was 0.2 mol/L phosphate buffer (pH8.5) containing 0.4 mol/L NaCl. The 0.2 mol/L boric acid was the most effective eluent. After purification, the contents of polysaccharide and protein were increased 66% and 2.65-fold, respectively. The protein-saccharide ratio was raised from 0.31 to 0.69.