文献类型：期刊文献

中文题名：吐温-85对螺旋霉素发酵过程的影响

英文题名：Effects of Tween-85 on the Spiramycin Fermentation

作者：朱凤[1];陈长华[1];张琪[2]

机构：[1]国家生物反应器工程国家重点实验室,华东理工大学生物工程学院,上海200237;[2]河南天方药业股份有限公司,河南驻马店463000

年份：2008

卷号：39

期号：3

起止页码：176

中文期刊名：中国医药工业杂志

外文期刊名：Chinese Journal of Pharmaceuticals

收录：CSTPCD;;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：螺旋霉素;吐温-85;效价

外文关键词：spiramycin; Tween-85; titers

摘要：研究了在培养基中添加吐温-85对发酵生产螺旋霉素的影响。结果表明:0h添加100mg/L吐温-85,发酵效价达28176u/ml,比对照提高11.9%。检测表明吐温-85可以增加细胞膜的通透性,提高乙酸激酶、酰基CoA合成酶和碱性磷酸酶的酶活力,并促进菌体对油的利用,提供更多的前体,从而提高螺旋霉素的产量。
The biosynthesis of spiramycin by Streptomyces ambofaciens by addition of Tween-85 to medium in 15L fermentor was studied. The results showed that the production achieved to 28176u/ml, which was increased by 11.9% over the control group, when 100mg/L Tween-85 was added into the medium. The mechanism of Tween-85 on spiramycin fermentation was also studied. The membrane permeability was increased and three enzymes acetate kinase, acyl-CoA synthetase and alkaline phosphatase, were activated and more oil could be utilized to provide more precursors so that more spiramycin was obtained.