文献类型：专利

英文题名：Preparation of optical pure D-type beta-hydroxy by providing resting cells of Gluconobacter oxydans, and converting substrate 2-methyl-1, 3-propanediol with resting cells

作者：WEI D;LIN J;WEI G;YANG X;WEI L;ZHOU Y;YIN B

机构：[1]UNIV EAST CHINA SCI & TECHNOLOGY

申请号：CN101280325-A

申请日：2008-05-23

公开日：2008-10-08

语种：英文

收录：DERWENT

摘要：NOVELTY - An optical pure D-type beta -hydroxy acid is prepared by providing resting cells of Gluconobacter oxydans; and converting substrate 2-methyl-1, 3-propanediol with resting cells to obtain desired product. The providing of resting cells of G. oxydans involves cultivating to obtain cell zymotic fluid of G. oxydans; centrifuging zymotic fluid to obtain thalli sediment; washing thalli using physiological saline; centrifugally removing supernatant; and suspending using phosphate buffer thalli to obtain the resting cells. USE - Method for preparing optical pure D-type beta -hydroxy acid (claimed). ADVANTAGE - The method realizes bioconversion under high cell density, thus saving energy and production costs.