文献类型：期刊文献

中文题名：复合载体固定化酵母生物合成胞二磷酸胆碱

英文题名：Biosynthesis of CDPC by Mixed Supports Immolibized Yeasts

作者：余冬生[1];邱蔚然[2];张灏[3]

机构：[1]安徽机电学院生化工程系,安徽芜湖241000;[2]华东理工大学生物反应器工程国家重点实验室,上海200237;[3]江南大学食品学院,江苏无锡214036

年份：2002

卷号：21

期号：3

起止页码：277

中文期刊名：无锡轻工大学学报（食品与生物技术）

外文期刊名：Journal of Wuxi University of Light Industry

收录：北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：复合载体;固定化;酵母;生物合成;胞二磷酸胆碱;核酸药物

外文关键词：CDPC; immobilized yeasts; orthogonal experiment

摘要：研究了复合载体固定酵母的条件 ,通过正交实验确定了K 卡拉胶 ,魔芋多糖和去离子水用量 .用扫描电子显微镜观察了固定化酵母冷冻前后的形态变化 ,固定化酵母多次使用后 ,其生物合成胞二磷酸胆碱 (CDPC)
The conditions of entrapping yeasts by mixed supports, k carrageenan and konjac glucomannan were studied.Through orthogonal experiments,the amount of k carrageenan, konjac glucomannan and deionized water were determined. The shape of immobilized yeasts was observed by scanning electron microscope, which was different between immobilized yeasts and frozen immobilized yeasts.High activity of immobilized yeasts was shown after they were used several times for biosynthesis of CDPC.