文献类型：期刊文献

英文题名：Overexpression of Capsular Polysaccharide Biosynthesis Protein in Lactobacillus plantarum P1 to Enhance Capsular Polysaccharide Production for Di-n-butyl Phthalate Adsorption

作者：Liu, Wei-Bing[1];Lin, Zhi-Wei[1];Zhou, Ying[1];Ye, Bang-Ce[1]

机构：[1]East China Univ Sci & Technol, Biomed Nanotechnol Ctr, Lab Biosyst & Microanal, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2021

卷号：31

期号：11

起止页码：1545

外文期刊名：JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000744227800009)】；

基金：This work was supported by grants from the National Natural Science Foundation of China (31730004) , and the General Program of Shanghai Natural Science Foundation (19ZR1413700) .

语种：英文

外文关键词：Lactobacillus plantarum; exopolysaccharides; probiotics; overexpression; di-n-butyl phthalate; adsorption

摘要：Exopolysaccharides (EPSs) such as capsular polysaccharide (CPS) are important bioactive carbohydrate compounds and are often used as bioenrichment agents and bioabsorbers to remove environmental pollutants like di-n-butyl phthalate (DBP). Among the EPS-producing bacteria, lactic acid bacteria (LAB) have gained the most attention. As generally recognized as safe (GRAS) microorganisms, LAB can produce EPSs having many different structures and no health risks. However, EPS production by LAB does not meet the needs of large-scale application on an industrial scale. Here, the capA gene (encoding CPS biosynthesis protein) was overexpressed in Lactobacillus plantarum P1 to improve the production of EPSs and further enhance the DBP adsorption capability. Compared with P1, the CPS production in capA overexpressed strain was increased by 11.3 mg/l, and the EPS thickness was increased from 0.0786 +/- 0.0224 mu m in P1 to 0.1160 +/- 0.0480 mu m in P1-capA. These increases caused the DBP adsorption ratio of P1-capA to be doubled. Overall, the findings in this study provide a safe method for the adsorption and removal of DBP.