文献类型：期刊文献

英文题名：Synthetic ePTFE Grafts Coated with an Anti-CD133 Antibody-Functionalized Heparin/Collagen Multilayer with Rapid in vivo Endothelialization Properties

作者：Lu, Shuyang[1,2];Zhang, Peng[4];Sun, Xiaoning[1,2];Gong, Feirong[4];Yang, Shouguo[1,2];Shen, Li[1,3];Huang, Zheyong[1,3];Wang, Chunsheng[1,2]

机构：[1]Fudan Univ, Shanghai Inst Cardiovasc Dis, Zhongshan Hosp, Shanghai 200032, Peoples R China;[2]Fudan Univ, Dept Cardiovasc Surg, Zhongshan Hosp, Shanghai 200032, Peoples R China;[3]Fudan Univ, Dept Cardiol, Zhongshan Hosp, Shanghai 200032, Peoples R China;[4]E China Univ Sci & Technol, Sch Mat Sci & Engn, Shanghai 200237, Peoples R China

年份：2013

卷号：5

期号：15

起止页码：7360

外文期刊名：ACS APPLIED MATERIALS & INTERFACES

收录：;EI(收录号：20133516667892);WOS:【SCI-EXPANDED(收录号:WOS:000323241100081)】；

基金：This work was supported by National Natural Science Foundation of China (grant 81000105). We extend appreciation to Dr. Jianguo Jia, Dr. Bingyu Li, Prof. Keqiang Wang, and Prof. Xiangdong Yang for technical support and expertise.

语种：英文

外文关键词：ePTFE grafts; surface modification; endothelialization; endothelial cells; platelet adhesion

摘要：An anti-CD133 antibody multilayer functionalized by heparin/collagen on an expanded polytetrafluoroethylene (ePTFE) graft was developed to accelerate early endothelializaton. The surface modification of ePTFE grafts demonstrated that the multilayer is stable in static incubation and shaking conditions and that the anti-CD133 antibodies were successfully cross-linked onto the surface. Blood compatibility tests revealed that the coimmobilized heparin/collagen films in the presence or absence of anti-CD133 antibodies prolonged the blood coagulation time and that there was less platelet activation and aggregation, whereas the hemolysis rate was comparable with the bare ePTFE grafts. Cellular proliferation was not inhibited, as the heparin/collagen synthetic vascular grafts coated with CD133 antibody showed little cytotoxicity. The endothelial cells adhered well to the modified ePTFE grafts during a cell adhesion assay. A porcine carotid artery transplantation model was used to evaluate the modified ePTFE grafts in vivo. The results of histopathological staining and scanning electron microscopy indicated that the anti-CD133 antibody was able to accelerate the attachment of vascular endothelial cells onto the ePTFE grafts, resulting in early rapid endothelialization. The success of the anti-CD133 antibody-functionalized heparin/collagen multilayer will provide an effective selection system for the surface modification of synthetic vascular grafts and improve their use in clinical applications.