文献类型：期刊文献

英文题名：Effects of porogens on the morphology and enantioselectivity of core-shell molecularly imprinted polymers with ursodeoxycholic acid

作者：Liang, Shuang[1];Wan, Junfen[1];Zhu, Jianliang[1];Cao, Xuejun[1]

机构：[1]E China Univ Sci & Technol, Dept Biochem Engn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2010

卷号：72

期号：2

起止页码：208

外文期刊名：SEPARATION AND PURIFICATION TECHNOLOGY

收录：;EI(收录号：20101312806802);WOS:【SCI-EXPANDED(收录号:WOS:000277640700012)】；

基金：This project was supported by Natural Scientific Foundation of China (20676032).

语种：英文

外文关键词：Molecularly imprinted polymers; Porogens; UDCA; Enantioselectivity

摘要：Porous core-shell molecularly imprinted polymers (MIPs) for ursodeoxycholic acid (UDCA) were prepared using acrylamide (AM) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linker. Molecular simulation program was used to calculate the interaction energy (Delta E) of monomer-template complex with and without porogens. The effect of porogens on the morphology and enantioselectivity of UDCA-imprinted polymers was investigated. Results showed that the existence and type of porogens influenced the ability of the particles to rebind UDCA. When liquid solvents of toluene, dodecyl alcohol/cyclohexanol (1/2, v/v), and acetone were used as porogens, toluene showed the best enantioselectivity to UDCA with separation factor (alpha) 3.24 and the largest specific surface area 158.83 m(2)/g, which was consistent with the molecular simulation results. As the solid granules of CaCO3 and Na2SO4 as porogens, CaCO3 exhibited better adsorption behavior and larger specific surface area than Na2SO4. In addition, a novel MIP was synthesized with solid granules of CaCO3 and liquid solvent of toluene as co-porogen. Comparing with the MIP using organic solvents or solid granules as a porogen, the improved MIP showed larger specific surface areas and pore volumes. The MIP with co-porogen also had good dynamic adsorption capacity (6.5 +/- 0.1 mg/ml). The MIP with co-porogen was used for the chromatographic separation of UDCA and its enantiomer chenodeoxycholic acid (CDCA) with separation factor 1.93. (C) 2010 Elsevier B.V. All rights reserved.