文献类型：期刊文献

英文题名：OPTIMUM INDUCTION OF RECOMBINANT THYMIDINE PHOSPHORYLASE AND ITS APPLICATION

作者：Ding, Qingbao[1];Ou, Ling[1];Wei, Dongzhi[1];Wei, Xiaokun[2]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Puchen Biotechnol Co Ltd, Shanghai, Peoples R China

年份：2011

卷号：30

期号：5

起止页码：360

外文期刊名：NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000294553500004)】；

语种：英文

外文关键词：thymidine phosphorylase; lactose; thymidine; deoxynucleoside

摘要：Recombinant E. coli pDEOA was constructed and lactose can be used instead of IPTG to induce the expression of thymidine phosphorylase by pDEOA. The use of lactose at concentrations higher than 0.5 mmol/L had an induction effect similar to that of IPTG but resulted in a longer initial induction time and better cell growth. The thymidine phosphorylase induced by lactose was very stable at 50 degrees C. Intact pDEOA cells induced by lactose can be used as a source of thymidine phosphorylase. Under standard reaction conditions, several deoxynucleosides were effectively produced from thymidine.